Methylation status of the reelin promoter region in the brain of schizophrenic patients
Methylation status of the reelin promoter region in the brain of schizophrenic patients
复制标题
DOI：
10.1016/j.biopsych.2007.07.003
复制
发表时间：
2008-03-01
影响因子：
10.6
通讯作者：
Kato, Tadafumi
中科院分区：
文献类型：
--
作者：
Tochigi, Mamoru;Iwamoto, Kazuya;Kato, Tadafumi
背景资料：在精神分裂症的病理生理学中，reelin（realelin，REALIN）启动子区域的超甲基化及其信使RNA和蛋白质水平的降低有牵连。方法：采用焦磷酸测序法检测14例精神分裂症患者和13例正常对照者的前额叶皮层中的cDNA-N启动子区的DNA甲基化状态。结果：14例精神分裂症患者的cDNA-N启动子区的DNA甲基化水平明显高于正常对照者，而13例正常对照者的cDNA-N启动子区的DNA甲基化水平明显低于正常对照者。分析的所有CpG和两个提出的CpNpG位点在对照受试者和精神分裂症患者中都没有检测到DNA甲基化（< 5%）。在灰色和白色物质中均未观察到可检测的DNA甲基化，排除了起始材料细胞异质性的可能性。结论：我们没有证实精神分裂症患者大脑中JNK启动子区域的超甲基化，这在以前的研究中提出。
Background: Hypermethylation of the reelin (RELN) promoter region and the reduced levels of its messenger RNA and protein have been implicated in the pathophysiology of schizophrenia. We intended a technical replication of recent studies that observed hypermethylation of CpG or CpNpG sites in the RELN promoter region in the brain of schizophrenic patients.Methods: The DNA methylation status of the promoter region of RELN was examined by using the pyrosequencing method in the prefrontal cortices of 14 patients with schizophrenia and 13 control subjects.Results: All of the CpG and two proposed CpNpG sites analyzed showed no detectable DNA methylation (< 5%) in both control subjects and patients with schizophrenia. No detectable DNA methylation was observed in both gray and white matter, excluding the possibility of cellular heterogeneity of start materials.Conclusions: We did not confirm the hypermethylation of the RELN promoter region in the brains of schizophrenic patients, suggested in the previous studies.