Allele frequency for c.335 A>C polymorphisms in porcine ghrelin/obestatin prepropeptide gene and association analysis with performance traits in various pig breeds
Allele frequency for c.335 A>C polymorphisms in porcine ghrelin/obestatin prepropeptide gene and association analysis with performance traits in various pig breeds
复制标题
猪ghrelin/obestatin前原肽基因c.335 A>C多态性等位基因频率及其与各猪品种生产性能的关联分析
DOI：
10.17221/8459-cjas
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发表时间：
2016
影响因子：
1.2
通讯作者：
Kensuke Hirose
中科院分区：
文献类型：
--
作者：
Kazuaki Tanaka;T. Takizawa;O. Oki;K. Fukawa;Tetsuya Ito;M. Miyabe;H. Mannen;Y. Kurosawa;Kensuke Hirose
对6个猪品种和2个野猪亚种的猪生长素释放肽/肥胖抑制素前原肽(GHRL)基因c.335A>C多态性等位基因频率进行了调查。 c.335C 是伯克夏猪、长白猪、大白猪、约克夏猪和克氏小型猪以及琉球野猪 (Sus scrofa riukiuanus) 中最常见的等位基因，而 c.335A 是杜洛克猪、眉山猪和日本野猪 (S. s. leucomystax) 中最常见的等位基因。在杜洛克、长白猪、大白猪和（长白×大白）×杜洛克（LWD）杂交猪中分析了 c.335A>C 与生产性状的关联。未检测到 c.335A>C 基因型与平均每日体重增加、背膘厚度或肌内脂肪之间的关联。然而，在杜洛克后备母猪中观察到腰部眼肌面积 (EMA) 与 c.335A>C 基因型之间存在关联。杜洛克后备母猪中，AA基因型组的EMA比AC基因型组大；然而，这种关联在杜洛克公猪和阉猪或其他调查的猪群中并不显着。这些结果表明，GHRL c.335A>C 不是猪 13 号染色体上影响脂肪沉积的主要数量性状候选基因座。
The allelic frequency of c.335A>C polymorphisms in the porcine ghrelin/obestatin prepropeptide (GHRL) gene was surveyed among six pig breeds and two subspecies of wild boars. The c.335C was the most frequent allele in Berkshire, Landrace, Large White, Yorkshire, and Clawn miniature pigs and Ryukyu wild boars (Sus scrofa riukiuanus), whereas c.335A was the most frequent allele in Duroc and Meishan pigs and Japanese wild boars (S. s. leucomystax). The association of c.335A>C with performance traits was analyzed in Duroc, Landrace, Large White, and (Landrace × Large White) × Duroc (LWD) cross-bred pigs. No associations between c.335A>C genotype and average daily weight gain, backfat thickness, or intramuscular fat were detected. However, an association was observed between loin eye muscle area (EMA) and c.335A>C genotype in Duroc gilts. The AA genotype group had larger EMA than the AC genotype group in Duroc gilts; however, this association was not significant in Duroc boars and barrows or the other pig populations investigated. These results demonstrate that GHRL c.335A>C is not a major quantitative trait loci candidate on pig chromosome 13 affecting fat deposition.