Anomalous immunostaining of 'optically clear' nuclei in gestational endometrium. A potential pitfall in the diagnosis of pregnancy-related herpesvirus infection.
Anomalous immunostaining of 'optically clear' nuclei in gestational endometrium. A potential pitfall in the diagnosis of pregnancy-related herpesvirus infection.
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妊娠子宫内膜“光学透明”细胞核的异常免疫染色。
DOI：
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发表时间：
1994
影响因子：
4.6
通讯作者：
P. di Sant’agnese
中科院分区：
文献类型：
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作者：
J. Sickel;P. di Sant’agnese
在妊娠期子宫内膜上皮中观察到一种独特的形态学改变，最初被描述为细胞核“透明化”。受影响的细胞核有一个清晰的中心和边缘染色质的边缘，赋予一个“玻璃”的外观让人想起疱疹病毒包涵体。我们在妊娠期子宫内膜中观察到这种变化，而妊娠期子宫内膜附着在正常足月胎盘的膜上。由于疱疹病毒感染的形态学提示，使用抗生物素蛋白-生物素法进行免疫过氧化物酶研究，导致核包涵体的强烈染色。在阴性对照中也观察到相同的核染色模式。链霉亲和素-生物素法复制了这种异常染色反应，但过氧化物酶-抗过氧化物酶法未复制。此外，在用游离抗生物素蛋白（0.05%）和游离生物素（0.05%）连续预孵育后，细胞核染色消失。使用过氧化物酶-抗过氧化物酶法，用生物素单克隆抗体鉴定阳性核染色。在另外9例与足月胎盘相关的光学透明核中获得了类似的结果。我们的结论是，染色的光学透明的细胞核是亲和素和链霉亲和素结合到核内生物素的结果。这种异常的染色模式可能导致疱疹病毒感染的误诊。
A distinctive morphologic alteration originally described as nuclear "clearing" has been observed in endometrial epithelium during pregnancy. Affected nuclei have a clear center and a rim of marginated chromatin, imparting a "glassy" appearance reminiscent of herpesvirus inclusions. We observed this change in gestational endometrium attached to the membranes of an otherwise normal-term placenta. Because of the morphologic suggestion of herpesvirus infection, immunoperoxidase studies using the avidin-biotin method were performed, resulting in intense staining of nuclear inclusions. An identical pattern of nuclear staining was also noted in negative controls. This anomalous staining reaction was replicated with the streptavidin-biotin method but not with the peroxidase-antiperoxidase method. Furthermore, nuclear staining was abolished after sequential preincubation with free avidin (0.05%) and free biotin (0.05%). Using the peroxidase-antiperoxidase method, positive nuclear staining was identified with monoclonal antibodies to biotin. Similar results were obtained in nine additional cases of optically clear nuclei associated with term placentas. We conclude that staining of optically clear nuclei is the result of avidin and streptavidin binding to intranuclear biotin. This anomalous staining pattern could potentially result in the misdiagnosis of herpesvirus infection.