Novel tnpR-based transposable promoter traps suitable for RIVET studies in different gram-negative bacteria
Novel tnpR-based transposable promoter traps suitable for RIVET studies in different gram-negative bacteria
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DOI：
10.1016/j.mimet.2013.01.017
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发表时间：
2013-04-01
影响因子：
2.2
通讯作者：
Lagares, Antonio
中科院分区：
文献类型：
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作者：
Lozano, Mauricio J.;Eugenia Salas, Maria;Lagares, Antonio
当需要高基因组覆盖率时，质粒携带的RIVET文库的制备可能会很麻烦。我们在此介绍了一组新的miniTn5启动子陷阱的构建和功能验证，以生成基于tnpr的RIVET库。通过转位产生tnpR转录融合的能力将极大地促进在Tn5转位起作用的细菌中建立RIVET研究。(C) 2013 Elsevier B.V.版权所有
The preparation of plasmid-borne RIVET libraries can be troublesome when high genomic coverages are needed. We present here the construction and functional validation of a new set of miniTn5 promoter traps to generate tnpR-based RIVET libraries. The ability to generate tnpR transcriptional fusions by transposition will significantly facilitate the setup of RIVET studies in those bacteria where Tn5 transposition is operative. (C) 2013 Elsevier B.V. All rights reserved.