Isolation and pathogenicity of pathogens from skin ulceration disease and viscera ejection syndrome of the sea cucumber Apostichopus japonicuss
Isolation and pathogenicity of pathogens from skin ulceration disease and viscera ejection syndrome of the sea cucumber Apostichopus japonicuss
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DOI：
10.1016/j.aquaculture.2008.10.015
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发表时间：
2009-02-01
期刊：
影响因子：
4.5
通讯作者：
Liu, Weidong
中科院分区：
文献类型：
--
作者：
Deng, Huan;He, Chongbo;Liu, Weidong
养殖海参病害已成为制约中国刺参养殖业可持续发展的瓶颈。2004年发生的导致海参大规模死亡的两种病害在辽宁和山东中国仍有流行。一种是技能溃疡综合征，另一种是内脏排斥综合征。对该病的流行病学和致病性进行了调查。对海参健康个体和患病个体进行显微观察，记录体壁表面的细菌、霉菌和寄生虫。以及微生物与技能溃烂病和脏腑排出病的关系。这两种疾病表现出几点不同。从室内越冬池塘和室外池塘养殖的海参中分离到6株优势菌株(2004A、2004B、04101、04102、04103和04104)。从室内越冬池塘内患内脏排出综合征的海参中分离到2株细菌(041201和041202)。对这些细菌的诱导感染实验表明，8个优势菌株均为致病菌株，表现出与自然感染相同的症状，而病参再分离株具有相同的形态特征。生理生化试验和16SRRNA基因序列相似性分析表明，菌株2004A与环状弧菌相似，菌株2004B与华支弧菌相似，菌株04101和04103与哈维氏弧菌相似，菌株04102与塔斯马尼亚弧菌相似，菌株04104与光细菌素相似。分离到与原节杆菌相似的041201株，与马葡萄球菌相似的041202株。药敏试验表明，8株细菌对氧氟沙星、盐酸左氧氟沙星、头孢比特敏感。强力霉素、新诺比星，但对苯唑西林钠、氨基青霉素、壮观霉素或头孢拉定不敏感。(C)2008爱思唯尔B.V.保留所有权利。
The diseases of cultured sea cucumber have been the bottle-neck for the Sustainable development of sea cucumber (Apostichopus jpaonicus) cultivation in China. Two diseases that Occurred from 2004 and caused mass mortalities of sea cucumbers are still prevalent in Liaoning and Shandong provinces in China. One is skill ulceration syndrome and the other is viscera rejection syndrome. The epidemiology and pathogenicity of the diseases were investigated. Microscopic observations were made oil healthy and diseased individuals of sea cucumbers to record the bacteria, mold and parasites on the surface of body wall. and the relationship of microbes between skill ulceration disease and viscera ejection disease. The two diseases expressed several differences. Six predominant isolates of bacteria (2004A, 2004B, 04101, 04102, 04103 and 04104) were isolated from the sea Cucumbers Suffering from skin ulceration in indoor ponds for wintering and outdoor ponds for Culturing. Two isolates of bacteria (041201 and 041202) were isolated from the sea cucumbers suffering from viscera ejection syndrome in indoor wintering ponds. Induced infection experiments with these isolates of bacteria showed that all eight predominant isolates of bacteria were pathogenic as they showed the same signs as naturally infected and re-isolates from diseased sea Cucumber had the same morphological characteristics. Physiological and biochemical tests and the sequence similarity analysis of the 16S rRNA genes of these bacterial isolates indicated that isolate 2004A was similar to Vibrio cyclitrophicus, 2004B similar to V. splendidus, isolates 04101 and 04103 similar to V. harveyi, 04102 similar to V. tasmaniensis, isolate 04104 similar to Photobacteriurn sp. isolate 041201 similar to Arthrobacter protophormiae, and isolate 041202 similar to Staphylococcus equorum. Antibiotic sensitive test showed that the eight isolates of bacteria were sensitive to Ofloxacin, Levofloxacin hydrochloride, Cefobid. Doxycycline, Novobiocin, but not sensitive to Oxacillin sodium, Aminobenzylpenicillin, Spectinomycin, or Cefradine. (c) 2008 Elsevier B.V. All rights reserved.