Antitumor Effects of Ganoderma Lucidum
Antitumor Effects of Ganoderma Lucidum
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灵芝的抗肿瘤作用
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
Kuang Y. Chen
中科院分区：
文献类型：
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作者：
Shiuh;Yau;Chieh‐fu Chen;Shenglin Wang;Kuang Y. Chen
灵芝是一种抗肿瘤的天然产物。用热水浸提法提取G.灵芝多糖（GLP）对C_3H小鼠纤维肉瘤有明显的抗肿瘤作用，并能抑制肿瘤的肺转移。此外，我们将GLP分级为多糖级分[GLP（AI）]和非多糖级分。我们发现GLP（AI）是显示C3 H小鼠纤维肉瘤生长的体内抗肿瘤死亡的主要成分。本实验观察了灵芝多糖（AI）对正常小鼠和荷瘤小鼠脾自然杀伤细胞（NK）杀伤活性的影响。结果表明，GLP（AI）经静脉、腹腔或口服给药，均可使不同品系小鼠脾脏NK活性呈剂量相关性增高。此外，GLP（AI）给药导致血清干扰素（IFN）滴度增加。此外，GLP（AI）治疗恢复了肿瘤听力小鼠脾脏NK细胞毒性的降低。还检查了GLP（AI）对白血病U937细胞中的分化诱导的作用。我们发现它能刺激血单个核细胞分泌细胞因子，这些细胞因子对白血病U937细胞既有抗增殖作用，又有诱导分化作用。
此外，G.灵芝对C57 BL/6小鼠腹腔接种刘易斯肺癌的抑制作用。结果表明，GLP单独或与细胞毒抗肿瘤药物（阿霉素、氟尿嘧啶、硫鸟嘌呤、甲氨蝶呤、顺铂）或合成免疫调节剂（imexon）联合腹腔给药可显著延长荷瘤小鼠的生存期。GLP对体外培养的细胞无细胞毒性，经环孢素预处理后，其抗肿瘤活性消失。发现活性成分主要存在于GLP（AI）级分中。
Ganoderma lucidum has been established to be an autitumor natural product. The hot water extracts of the mycelium of G. lucidum (GLP) exhibited antitumor effect against fibrosarcoma in male and female C3H mice and inhibited the metastasis of the tumor to the lung. Moreover, we have fractionated GLP into polysaccharide fraction [GLP(AI)] and non-polysaccharide fraction. We found that GLP (AI) is the major component to show the in vivo antitumor died on fibrosarcoma growth in C3H mice. The effects of GLP (AI) on cytotoxic activity of splenic natural killer cells (NK) were assessed in normal mice and in tumor hearing mice. The rsu1ts show that either intravenous, intraperitoneal or oral administration of GLP (AI) produced a dose-related increase in the splenic NK activity in different mouse strains. In addition, administration of GLP (AI) produced an increase in the titer of serum intertron (IFN). Furthermore, the decreased splenic NK cytotoxicity of tumor-hearing mice was restored by GLP (AI) treatment. The effect of GLP (AI) on the induction of differentiation in leukemic U937 cells was also examined. We found that it could stimulate blood mononuclear cells to secrete cytokines which were both anti-proliferative and differentiation inductive to the leukemnic U937 cells.
Furthermore, antitumor activity of G. lucidum on intraperitoneally implanted Lewis lung carcinoma in synergenic C57BL/6 mice was investigated. The results showed that GLP significantly increasd the life-span of tumor-implanted mice, when administered intraperitoneally alone or in combination with cytotoxic antitumor drugs (adriamycin, fluorouracil, thioguanine, methotrexate, cisplatin) or synthetic immunomodulator (imexon). The GLP was not cytotoxic to cultured cells and the antitumor activity was abolished by pretreatment of mice with cyclosporine. The active principle (s) was found to be present predominantly in the GLP (AI) fraction.