T-box genes in the ascidian Ciona intestinalis:: Characterization of cDNAs and spatial expression
T-box genes in the ascidian Ciona intestinalis:: Characterization of cDNAs and spatial expression
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DOI：
10.1002/dvdy.20082
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发表时间：
2004-08-01
影响因子：
2.5
通讯作者：
Takahashi, H
中科院分区：
文献类型：
--
作者：
Takatori, N;Hotta, K;Takahashi, H
T-box转录因子家族的成员共享一个进化保守的DNA结合域，在胚胎发育的各种过程中发挥着重要的作用。根据序列相似性，脊椎动物T-box基因分为5个亚家族(8组)：Brachyly、Tbx1(Tbx1/10、Tbx15/18/22、Tbx20)、Tbx2/3/4/5(Tbx2/3和Tbx4/5)、Tbx6和Tbr/Eome/Tbx21。海鞘是原始的脊索动物，它们的蝌蚪幼虫被认为代表了脊椎动物的简化和基本的身体结构。此外，还发现海鞘基因组包含与发育有关的基因的基本祖先补充。目前的cDNA特征和对Ciona enterinalsDw基因组的研究表明，Ciona基因组中包含一个拷贝的基因，每个拷贝基因对应于短尾藻Tbx1/10、Tbx15/18/22、Tbx20、Tbx2/3和Tbr/Eome/Tbx21组，以及至少三个拷贝的Tbx6亚家族。每个Ciona T-box基因都表现出一种特有的表达模式，尽管Tbx20的表达模式在本研究中没有确定。这些结果将为未来研究每个基因的功能，不同T-box基因的遗传级联，以及在这个原始脊索中T-box基因结构和组织的进化变化的全基因组调查提供有用的基本信息。(C)2004年Wiley-Liss公司
Members of the T-box family of transcription factors share an evolutionarily conserved DNA-binding domain and play significant roles in various processes of embryonic development. Vertebrate T-box genes are categorized into the following five major subfamilies (eight groups), depending on sequence similarities: Brachyuly, Tbx1 (Tbx1/10, Tbx15/18/22, Tbx20), Tbx2/3/4/5 (Tbx2/3 and Tbx4/5), Tbx6, and Tbr/Eomes/TBX21. Ascidians are primitive chordates, and their tadpole larva are considered to represent the simplified and basic body plan of vertebrates. In addition, it has been revealed that the ascidian genome contains the basic ancestral complement of genes involved in development. The present characterization of cDNAs and survey of the Ciona intestinalis draft genome demonstrated that the Ciona genome contains a single copy gene for each of the Brachyury, Tbx1/10, Tbx15/18/22, Tbx20, Tbx2/3, and Tbr/Eomes/TBX21 groups, and at least three copies of the Tbx6 subfamily. Each of the Ciona T-box genes shows a characteristic expression pattern, although that of Tbx20 was not determined in the present study. These results provide basic information that will be useful for future studies of the function of each gene, genetic cascades of different T-box genes, and genome-wide surveys of evolutionary changes in the T-box gene structure and organization in this primitive chordate. (C) 2004 Wiley-Liss, Inc.