The CD24+ cell subset promotes invasion and metastasis in human osteosarcoma
The CD24+ cell subset promotes invasion and metastasis in human osteosarcoma
复制标题
CD24( )细胞亚群促进人骨肉瘤的侵袭和转移
DOI：
10.1016/j.ebiom.2019.102598
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发表时间：
2020-01-01
期刊：
影响因子：
11.1
通讯作者：
Xiao, Jianru
中科院分区：
文献类型：
--
作者：
Zhou, Zhenhua;Li, Yan;Xiao, Jianru
背景资料：骨肉瘤是影响儿童和青少年的最常见的原发性侵袭性骨肿瘤。转移瘤通常对常规化疗耐药，平均生存期短。通过鉴定和表征有助于其侵袭行为的分子，开发有价值的诊断指标和靶向药物将对临床诊断具有重要意义。方法：我们检测了骨肉瘤亲本细胞和球细胞中常见干细胞标记物的差异表达水平。此外，我们进一步分析了骨肉瘤细胞体外化疗前后候选常见干细胞标志物的变化。本研究系统研究了CD24（+）细胞在骨肉瘤中的增殖、迁移、侵袭、成瘤和转移等生物学功能，并分析了CD24（+）细胞与骨肉瘤患者预后的关系。通过敲低CD24来预防肿瘤的形成和转移，突出了CD24作为骨肉瘤治疗靶点的潜力。结论：CD24（+）细胞亚群在骨肉瘤的侵袭和转移中起重要作用。(C)2019年，任作家。由爱思唯尔公司出版
Background: Osteosarcoma is the most common primary aggressive bone tumor affecting children and young adolescents.Metastases are often resistant to conventional chemotherapy and mean short-term survival.Development of valuable diagnostic indicators and targeting agents will have important implications for clinical diagnosis by the identification and characterization of molecules that contribute to its aggressive behavior.Methods: We examined differential expression levels of common stem cell markers in osteosarcoma parental and sphere cells. In addition, we further analyzed the changes of candidate common stem cell markers before and after in vitro chemotherapy of osteosarcoma cells. The biological functions of CD24(+) subpopulation in osteosarcoma such as proliferation, migration, invasion, tumorigenesis and metastasis were systematically investigated, and the correlations of CD24 levels with prognosis in patients with osteosarcoma were analyzed.Findings: CD24(+) Cells presented characteristics of TICs and resist drug-induced apoptosis. The prevention of tumor formation and metastasis by CD24 knockdown highlights the potential of CD24 as a therapeutic target for osteosarcoma. Moreover, the levels of CD24 in osteosarcoma samples were significantly correlated with the prognosis of patients.Interpretation: CD24(+) cell subset played an important role in osteosarcoma invasion and metastasis. (C) 2019 The Authors. Published by Elsevier B.V.