The effect of rhG-CSF on the conformation of LFA-1 on CD4+ T cells in hemopoietic stem cell transplantation
The effect of rhG-CSF on the conformation of LFA-1 on CD4+ T cells in hemopoietic stem cell transplantation
复制标题
造血干细胞移植中rhG-CSF对CD4 T细胞上LFA-1构象的影响
DOI：
10.1080/08923970802530510
复制
发表时间：
2009-06-01
影响因子：
3.3
通讯作者：
Gao Chunji
中科院分区：
文献类型：
--
作者：
Chen Weihua;Wang Fei;Gao Chunji
重组人粒细胞集落刺激因子（rhG-CSF）在造血干细胞移植中调节供体T细胞功能。rhG-CSF对CD4+ T细胞活化的影响研究甚少。我们研究了rhG-CSF的动员是否影响CD4+ T细胞的活化和增殖能力。用phorbol 12-肉豆蔻酸13-乙酸酯（PMA）加离子霉素或CD3 mAb OKT3加细胞间细胞粘附分子-1 （ICAM-1）在37℃下处理6小时，细胞暴露CD25、CD69和MEM148表位显著增加。与动员前相比，rhG-CSF动员降低了活化CD4+ T细胞上CD25、CD69和MEM148表位的表达。转录因子Jun activation domain binding protein 1 （JAB1）在CD4+ T细胞的活化中起作用，rhG-CSF的动员改变了细胞核中JAB1蛋白的水平。rhG-CSF的动员也降低了CD4+ T细胞对ICAM-1的粘附，但对供体CD4+CD25+调节性T细胞的水平没有影响。总的来说，这些数据表明rhG-CSF的动员可以通过LFA-1/ICAM-1共刺激信号影响HSC移植中CD4+ T细胞的激活。
Recombinant human granulocyte colony-stimulating factor (rhG-CSF) modulates donor T cell function in hemopoietic stem cell transplantation. The effects of rhG-CSF on the activation of CD4+ T cells have been poorly investigated. We investigated whether rhG-CSF mobilization influenced the activation and proliferation capacity of CD4+ T cells. Cell treatment with phorbol 12-myristate 13-acetate (PMA) plus ionomycin or the CD3 mAb OKT3 plus intercellular cell adhesion molecule-1 (ICAM-1) at 37°C for 6 h induced a dramatic increase in CD25, CD69 and MEM148 epitope exposure. rhG-CSF mobilization decreased CD25, CD69 and MEM148 epitope expression on activated CD4+ T cells compared with cells before mobilization. The transcription factor Jun activation domain-binding protein 1 (JAB1) plays a role in the activation of CD4+ T cells, and the rhG-CSF mobilization changed the level of nuclear JAB1 protein. rhG-CSF mobilization also decreased the adhesion of CD4+ T cells to ICAM-1, but had no effect on the levels of donor CD4+CD25+ regulatory T cells. Overall, these data suggest the rhG-CSF mobilization can influence CD4+ T cell activation through LFA-1/ICAM-1 costimulatory signaling in HSC transplantation.