Microtubule-associated protein 1B: a neuronal binding partner for myelin-associated glycoprotein.
Microtubule-associated protein 1B: a neuronal binding partner for myelin-associated glycoprotein.
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DOI：
10.1083/jcb.200108137
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发表时间：
2001-12-10
期刊：
影响因子：
--
通讯作者：
Quarles RH
中科院分区：
文献类型：
--
作者：
Franzen R;Tanner SL;Dashiell SM;Rottkamp CA;Hammer JA;Quarles RH
髓磷脂相关糖蛋白（MAG）在髓鞘神经胶质细胞的轴突周膜中表达，据信它通过与轴突的成分结合而在神经胶质细胞-轴突相互作用中发挥作用。涉及蛋白质印迹叠加和免疫共沉淀的实验表明，MAG 与背根神经节神经元 (DRGN) 中表达的微管相关蛋白 1B (MAP1B) 的磷酸化神经元亚型和大脑有髓轴突的轴突富集部分结合，但不与神经胶质细胞表达的 MAP1B 亚型结合。一些 MAP1B 作为神经元质膜糖蛋白的表达（Tanner, S.L., R. Franzen, H. Jaffe, and R.H. Quarles. 2000. J. Neurochem. 75:553–562.），通过其无需细胞透化的免疫染色进一步记录，与它作为轴突表面上 MAG 的结合伴侣一致。 DRGN 上 MAG-Fc 嵌合体的结合位点与神经元静脉曲张上的 MAP1B 共定位，MAG 和 MAP1B 也共定位于有髓轴突的轴突周围区域。此外，当 DRGN 与 MAG 转染的 COS 细胞共培养时，MAP1B 磷酸化亚型的表达显着增加。 MAG 与 MAP1B 的相互作用与 MAG 在影响有髓轴突的细胞骨架结构和稳定性方面的已知作用相关。
Myelin-associated glycoprotein (MAG) is expressed in periaxonal membranes of myelinating glia where it is believed to function in glia–axon interactions by binding to a component of the axolemma. Experiments involving Western blot overlay and coimmunoprecipitation demonstrated that MAG binds to a phosphorylated neuronal isoform of microtubule-associated protein 1B (MAP1B) expressed in dorsal root ganglion neurons (DRGNs) and axolemma-enriched fractions from myelinated axons of brain, but not to the isoform of MAP1B expressed by glial cells. The expression of some MAP1B as a neuronal plasma membrane glycoprotein (Tanner, S.L., R. Franzen, H. Jaffe, and R.H. Quarles. 2000. J. Neurochem. 75:553–562.), further documented here by its immunostaining without cell permeabilization, is consistent with it being a binding partner for MAG on the axonal surface. Binding sites for a MAG-Fc chimera on DRGNs colocalized with MAP1B on neuronal varicosities, and MAG and MAP1B also colocalized in the periaxonal region of myelinated axons. In addition, expression of the phosphorylated isoform of MAP1B was increased significantly when DRGNs were cocultured with MAG-transfected COS cells. The interaction of MAG with MAP1B is relevant to the known role of MAG in affecting the cytoskeletal structure and stability of myelinated axons.
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影响因子：
2.9
作者：
Ma, DL;Chow, S;Fischer, I
通讯作者：
Fischer, I
影响因子：
4.2
作者：
SAPIRSTEIN, VS;DURRIE, R;FISCHER, I
通讯作者：
FISCHER, I
DOI：
10.1016/s0736-5748(98)00068-9
发表时间：
1999-04-01
影响因子：
1.8
作者：
Turnley, AM;Bartlett, PF
通讯作者：
Bartlett, PF
影响因子：
7.8
作者：
Tang, S;Shen, YJ;Filbin, MT
通讯作者：
Filbin, MT
影响因子：
5.3
作者：
Yin, XH;Crawford, TO;Trapp, BD
通讯作者：
Trapp, BD