Hybridization and reaction-based fluorogenic nucleic acid probes
Hybridization and reaction-based fluorogenic nucleic acid probes
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DOI：
10.1039/c2cc33827k
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发表时间：
2012-01-01
影响因子：
4.9
通讯作者：
Mokhir, Andriy
中科院分区：
文献类型：
--
作者：
Dutta, Subrata;Flottmann, Benjamin;Mokhir, Andriy
我们开发了荧光探针，在特定核酸模板存在的情况下，随着荧光染料的释放，这种探针可以被光激活。这种模板化反应可用于靶向复杂混合物中的特定核酸。我们进一步证明，这个反应可以通过单分子荧光成像进行监测。
We developed fluorogenic probes, which are photoactivated in the presence of specific nucleic acid templates with the release of fluorescent dyes. This templated reaction can be used to target specific nucleic acids in complex mixtures. We further demonstrate that this reaction can be monitored by single-molecule fluorescence imaging.