Fast and Efficient Mouse Pluripotency Reprogramming Using a Chemically-Defined Medium.
Fast and Efficient Mouse Pluripotency Reprogramming Using a Chemically-Defined Medium.
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DOI：
10.3390/mps5020028
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发表时间：
2022-03-24
影响因子：
2.4
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中科院分区：
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体细胞重组获得诱导多能干细胞是一项重要的生物学和医学突破，为再生医学和疾病模型等领域提供了重要的应用。然而，这项很有前途的技术由于效率低和动力学速度慢而受到阻碍。因此，我们建立了一种实用的工作流程，使用iCD1(iPS化学定义介质1)快速高效地从小鼠胚胎成纤维细胞(MEF)中诱导出IPSCs。该方案可以很容易地在标准细胞培养实验室中实施，并可应用于细胞命运研究。
The reprogramming of somatic cells to obtain induced pluripotent stem cells (iPSCs) is an important biological and medical breakthrough, providing important applications for fields such as regenerative medicine and disease modeling. However, this promising technology is damped due to its low efficiency and slow kinetics. Therefore, we generated a practical workflow to rapidly and efficiently induce iPSCs from mouse embryonic fibroblasts (MEFs) using iCD1 (iPS chemically-defined medium 1). This protocol can easily be implemented in a standard cell culture laboratory and be applied to cell fate research.