Wolbachia infections of phlebotomine sand flies (Diptera: Psychodidae)
Wolbachia infections of phlebotomine sand flies (Diptera: Psychodidae)
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DOI：
10.1603/0022-2585-38.2.237
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发表时间：
2001-03-01
影响因子：
2.1
通讯作者：
O'Neill, SL
中科院分区：
文献类型：
--
作者：
Ono, M;Braig, HR;O'Neill, SL
筛选了旧大陆和新大陆白蛉种感染沃尔巴克氏体，沃尔巴克氏体是在许多节肢动物和丝虫线虫中发现的细胞内细菌内共生体。在代表15个物种的53个样品中，通过使用Wolbachia表面蛋白（wsp）基因的引物进行聚合酶链反应扩增，发现4个物种的9个样品对Wolbachia呈阳性。从四个物种的wsi，基因片段的5个克隆，测序，并用于系统发育分析。这些wsp序列被放置在三个不同的分支内的节肢动物相关的沃尔巴克氏体（组A和B），表明沃尔巴克氏体感染白蛉在一个以上的场合。两种亲缘关系较远的沙蝇种Lutzomyia（Psanthromyia）shannoni（Dyar）和Lutzomyia（Nyssomyia）whitmani（安图内斯& Coutinho）感染了相同的沃尔巴克氏体菌株，表明最近的水平传播。
Old and New World phlebotomine sand fly species were screened for infection with Wolbachia, intracellular bacterial endosymbionts found in many arthropods and filarial nematodes. Of 53 samples representing 15 species, nine samples of four species were found positive for Wolbachia by polymerase chain reaction amplification using primers for the Wolbachia surface protein ((wsp) gene. Five of the wsi, gene fragments from four species were cloned, sequenced, and used for phylogenetic analysis. These wsp sequences were placed in three different clades within the arthropod associated Wolbachia (groups A and B), suggesting that Wolbachia has infected sand flies on more than one occasion. Two distantly related sand fly species, Lutzomyia (Psanthromyia) shannoni (Dyar) and Lutzomyia (Nyssomyia) whitmani (Antunes & Coutinho), infected with an identical Wolbachia strain suggest a very recent horizontal transmission.