T‐cell response to structural and nonstructural hepatitis C virus antigens in persistent and self‐limited hepatitis C virus infections
T‐cell response to structural and nonstructural hepatitis C virus antigens in persistent and self‐limited hepatitis C virus infections
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持续性和自限性丙型肝炎病毒感染中 T 细胞对结构性和非结构性丙型肝炎病毒抗原的反应
DOI：
10.1002/hep.1840190205
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发表时间：
1994
期刊：
影响因子：
13.5
通讯作者：
F. Fiaccadori
中科院分区：
文献类型：
--
作者：
C. Ferrari;A. Valli;L. Galati;A. Penna;Patrizia Scaccaglia;T. Giuberti;C. Schianchi;Gabriale Missale;M. Marin;F. Fiaccadori
研究了29例慢性丙型肝炎患者和15例临床上从丙型肝炎病毒感染中恢复的无症状丙型肝炎病毒抗体阳性受试者的外周血淋巴细胞对丙型肝炎病毒结构和非结构抗原的增殖反应（核心、包膜、非结构4和非结构5）在酵母中作为超氧化物歧化酶融合蛋白表达，在最初的尝试，以确定病毒特异性免疫反应的一些特征。丙型肝炎病毒核心是人类白细胞抗原II类限制性T细胞的免疫原性最强的抗原在两组患者的研究，它的增殖反应是最有力的，最频繁的表达相比，与其他抗原测试。结果的特异性得到了健康未感染对照对丙型肝炎病毒抗原缺乏应答的支持，并通过用酵母源核心刺激T细胞产生的多克隆T细胞系识别不同来源的重组核心蛋白（酵母和杆状病毒）得到证实。测试的每种抗原都能够诱导显著但可变水平的增殖应答，表明所有抗原在T细胞水平上都具有免疫原性。在能够根除感染的受试者中比慢性丙型肝炎患者更频繁地检测到对核心、非结构4和非结构5抗原的显著增殖反应，尽管差异在统计学上不显著。两组患者对推定包膜抗原的反应无差异。（Hepatology 1994;19：286-295）。
Twenty‐nine patients with chronic hepatitis C and 15 asymptomatic hepatitis C virus antibody–positive subjects who clinically recovered from hepatitis C virus infection were studied for their peripheral blood lymphomononuclear cell proliferative response to hepatitis C virus structural and nonstructural antigens (core, envelope, nonstructural 4 and nonstructural 5) expressed in yeast as superoxide dismutase fusion proteins, in an initial attempt to define some of the features of the virus‐specific immune response. Hepatitis C virus core was the most immunogenic antigen for human leukocyte antigen class II–restricted T cells in both groups of patients studied, and the proliferative response to it was the most vigorous and the most frequently expressed in comparison with the other antigens tested. The specificity of the results was supported by the lack of response to hepatitis C virus antigens by healthy uninfected controls and confirmed by recognition of recombinant core proteins of different origin (yeast and baculovirus) by polyclonal T‐cell lines produced by T‐cell stimulation with yeast‐derived core. Each of the antigens tested was able to induce significant although variable levels of proliferative response, indicating that all can be immunogenic at the T‐cell level. Significant proliferative responses to core, nonstructural 4 and nonstructural 5 antigens were more frequently detected in subjects who were able to eradicate infection than in patients with chronic hepatitis C, although the difference was statistically not significant. No difference was observed between the two groups of patients with respect to the response to the putative envelope antigens. (Hepatology 1994;19:286–295).