Complement-mediated phagocytosis - The role of Syk
Complement-mediated phagocytosis - The role of Syk
复制标题
DOI：
10.1080/15216540600746377
复制
发表时间：
2006-05-01
期刊：
影响因子：
4.6
通讯作者：
Yamamura, Hirohei
中科院分区：
文献类型：
--
作者：
Tohyama, Yumi;Yamamura, Hirohei
吞噬作用是先天免疫反应中的中心事件，由病原体表面的配体和吞噬细胞膜上的受体之间的结合而触发。特别是，补体介导的吞噬作用是通过吞噬细胞上相应的补体受体特异性识别结合的补体成分来完成的。蛋白酪氨酸激酶，Syk，在适应性免疫系统中Fcγ受体介导的吞噬过程中起着核心作用。最近使用巨噬细胞样分化细胞系和血清处理的酵母多糖的研究发现，Syk在补体介导的吞噬作用中也在先天性免疫中发挥重要作用。经血清处理的酵母多糖颗粒迅速附着在细胞上，随后通过补体受体3被吞噬。在这个过程中，Syk被酪氨酸磷酸化，并聚集在新生的吞噬小体周围。将Syk-siRNA或显性阴性Syk(dN-Syk)转移到巨噬细胞中会导致病原体的吞噬功能受损。总体而言，在补体介导的吞噬作用中，Syk是吞噬病原体所必需的。
Phagocytosis is a central event in the innate immune responses that are triggered by the association between ligands on the surface of pathogens and receptors on the membrane of phagocytes. Particularly, complement-mediated phagocytosis is accomplished by specific recognition of bound complement components by the corresponding complement receptors on the phagocytes. The protein-tyrosine kinase, Syk, plays a central role in Fc gamma receptor-mediated phagocytosis in the adaptive immune system. From recent studies using a macrophage-like differentiated cell line and serum-treated zymosan, it was found that Syk also plays an essential role in complement-mediated phagocytosis in innate immunity. Serum-treated zymosan particles promptly attached to the cells and were subsequently engulfed via complement receptor3. During this process, Syk became tyrosine-phosphorylated and accumulated around the nascent phagosomes. The transfer of Syk-siRNA or dominant-negative Syk (DN-Syk) into macrophages resulted in impaired engulfment of pathogen. Collectively, Syk is required for the engulfment of pathogen in complement-mediated phagocytosis.