Effects of Vasoactive Intestinal Polypeptide, Monoamines, Prostaglandins, and 2‐Chloroadenosine on Adenylate Cyclase in Rat Cerebral Microvessels
Effects of Vasoactive Intestinal Polypeptide, Monoamines, Prostaglandins, and 2‐Chloroadenosine on Adenylate Cyclase in Rat Cerebral Microvessels
复制标题
血管活性肠多肽、单胺、前列腺素和2-氯腺苷对大鼠脑微血管腺苷酸环化酶的影响
DOI：
10.1111/j.1471-4159.1983.tb08038.x
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发表时间：
1983
影响因子：
4.7
通讯作者：
O. Rorstad
中科院分区：
文献类型：
--
作者：
Minta Huang;O. Rorstad
摘要：从大鼠大脑皮层分离的微血管中的腺苷酸环化酶被鸟嘌呤核苷酸、儿茶酚胺、前列腺素E1、前列腺素E2和2-氯腺苷刺激。通过与β-肾上腺素能受体的相互作用介导儿茶酚胺刺激。相对效价顺序为：异丙肾上腺素>肾上腺素>去甲肾上腺素。微血管腺苷酸环化酶的激活与E1和E2以及2-氯腺苷的剂量相关。测试了22种肽对微血管腺苷酸环化酶的可能影响。只有血管活性肠肽（VIP）是刺激性的。未观察到抑制作用。VIP的激活需要三磷酸鸟苷，并且在10 nM至μM范围内具有剂量依赖性（ED 50 = 0.1 μM）。在30°C下，肽对腺苷酸环化酶的刺激随时间线性增加，最长可达15 min。VIP的作用不受酚妥拉明或普萘洛尔的抑制，表明其作用不是通过与α或β肾上腺素能受体相互作用引起的。VIP和异丙肾上腺素、前列腺素E1或2-氯腺苷实现的激活是个体刺激的总和，表明VIP的受体与异丙肾上腺素、前列腺素E1和2-氯腺苷的受体不同。
Abstract: Adenylate cyclase in microvessels isolated from rat cerebral cortex was stimulated by guanine nucleotides, catecholamines, prostaglandin E1, prostaglandin E2, and 2‐chloroadenosine. Catecholamine stimulation was mediated by interaction with β‐adrenergic receptors. The order of relative potency was: isoproterenol > epinephrine > norepinephrine. Activation of microvessel adenylate cyclase by prostaglandins E1 and E2 as well as by 2‐chloroadenosine was dose related. Twenty‐two peptides were tested for possible effects on the microvessel adenylate cyclase. Only vasoactive intestinal polypeptide (VIP) was stimulatory. No inhibitory action was observed. Activation by VIP required guanosine triphosphate and was dose dependent from 10 nM to μM (ED50= 0.1 μM). At 30°C, stimulation of adenylate cyclase by the peptide increased linearly with time for up to 15 min. The effect of VIP was not inhibited by phentolamine or propranolol, suggesting that its action was not elicited by interaction with α‐ or β‐adrenergic receptors. Activation achieved by VIP and isoproterenol, prostaglandin E1, or 2‐chloroadenosine was the sum of the individual stimulations, suggesting that receptors for VIP were distinct from those for isoproterenol, prostaglandin E1, and 2‐chloroadenosine.