Transposition of Ac from the P locus of maize into unreplicated chromosomal sites.
Transposition of Ac from the P locus of maize into unreplicated chromosomal sites.
复制标题
Ac 从玉米 P 基因座转座到未复制的染色体位点。
DOI：
10.1093/genetics/117.1.109
复制
发表时间：
1987
期刊：
影响因子：
3.3
通讯作者：
Dellaporta,SL
中科院分区：
文献类型：
--
作者：
Chen,J;Greenblatt,IM;Dellaporta,SL
我们已经分析了由于体细胞转座而改变的玉米可移动元件激活子(Ac)的供体和靶点。先前的遗传分析表明，由P转座产生的两个有丝分裂子系在P位点处的组成不同。然而，这两个谱系通常都含有映射到相同遗传位置的转位基因。利用甲基化敏感的限制性内切酶和基因组印迹分析，我们鉴定了供体Pocus和Ac靶点上的AcEles，并利用该方法克隆了Pocus并鉴定了转座的AcEles。女儿系被证明是来自单一转位事件的有丝分裂后代。当两个谱系都包含Acgenic活性时，它们都在相同的基因组片段上包含转位AcElement，而与目标位置无关。这表明，在大多数情况下，顶座置换发生在供体基因座复制之后，但在目标位置复制完成之前。
We have analyzed donor and target sites of the mobile elementActivator(Ac) that are altered as a result of somatic transposition from thePlocus in maize. Previous genetic analysis has indicated that the two mitotic daughter lineages which result fromActransposition fromPdiffer in theirAcconstitution at thePlocus. Both lineages, however, usually contain transposedAcelements which map to the same genetic position. Using methylation-sensitive restriction enzymes and genomic blot analysis, we identifiedAcelements at both the donorPlocus andActarget sites and used this assay to clone thePlocus and to identify transposedAcelements. Daughter lineages were shown to be mitotic descendants from a single transposition event. When both lineages containedAcgenetic activity, they both contained a transposedAcelement on identical genomic fragments independent of the genetic position of the target site. This indicates that in the majority of cases,Actransposition takes place after replication of the donor locus but before completion of replication at the target site.
细菌中的复制性和保守性转座
影响因子：
64.5
作者：
K. Derbyshire;N. Grindley
通讯作者：
N. Grindley
位点特异性甲基化对限制性内切酶消化的影响。
影响因子：
14.9
作者：
Michael McClelland;M. Nelson
通讯作者：
M. Nelson