Three-dimensional evaluation of the spinal local neural network revealed by the high-voltage electron microscopy: a double immunohistochemical study
Three-dimensional evaluation of the spinal local neural network revealed by the high-voltage electron microscopy: a double immunohistochemical study
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DOI：
10.1007/s00418-012-0976-6
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发表时间：
2012-10-01
影响因子：
2.3
通讯作者：
Sakamoto, Hirotaka
中科院分区：
文献类型：
--
作者：
Oti, Takumi;Satoh, Keita;Sakamoto, Hirotaka
解剖学超微结构的三维分析在生物学研究中具有重要意义。然而，从生物标本的超薄切片的精确的系列集上的3-D图像分析是非常难以实现的，并且由于可以重建的小区域，通过从这些切片的3-D重建可以获得有限的信息。另一方面，通过超高加速电压的电子的高穿透力使得能够检查生物样本的厚切片。高压电子显微镜（HVEM）对于中枢神经系统的三维分析特别有用，因为可以在超微结构水平上观察到相当厚的切片。在这里，我们应用HVEM断层扫描辅助光学显微镜研究的3-D化学神经解剖的大鼠下脊髓双标记免疫组织化学注释。这种强大的方法是有用的研究分子和/或化学神经解剖学在3-D超微结构水平。
Three-dimensional (3-D) analysis of anatomical ultrastructures is important in biological research. However, 3-D image analysis on exact serial sets of ultra-thin sections from biological specimens is very difficult to achieve, and limited information can be obtained by 3-D reconstruction from these sections due to the small area that can be reconstructed. On the other hand, the high-penetration power of electrons by an ultra-high accelerating voltage enables thick sections of biological specimens to be examined. High-voltage electron microscopy (HVEM) is particularly useful for 3-D analysis of the central nervous system because considerably thick sections can be observed at the ultrastructure level. Here, we applied HVEM tomography assisted by light microscopy to a study of the 3-D chemical neuroanatomy of the rat lower spinal cord annotated by double-labeling immunohistochemistry. This powerful methodology is useful for studying molecular and/or chemical neuroanatomy at the 3-D ultrastructural level.