A Robertsonian translocation from Thinopyrum bessarabicum into bread wheat confers high iron and zinc contents
A Robertsonian translocation from Thinopyrum bessarabicum into bread wheat confers high iron and zinc contents
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DOI：
10.1111/pbr.12359
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发表时间：
2016-06-01
期刊：
影响因子：
2
通讯作者：
Badakhshan, Hedyeh
中科院分区：
文献类型：
--
作者：
Ardalani, Somayeh;Mirzaghaderi, Ghader;Badakhshan, Hedyeh
小麦异源易位系的建立促进了异源物种在小麦改良中的实际利用。本文报道了小麦染色体6D和小麦染色体6E(B)(2n=2x=14，(EEB)-E-b)通过中心断裂-融合机制产生的补偿小麦-贝氏偃麦草全臂罗伯逊易位(Robt)。以面包小麦品种柔山‘(2n=6x=42，AABBDD)为母本，以6E(B)(6D)代换系为母本，以6D和6E(B)双单体植株为母本，获得了F-2群体。对80个F-2基因型(L1~L80)的染色体组成进行了筛选。利用6D和6E(B)染色体特异的3个标记基因(PLUG)对F-2代植株进行了筛选。鉴定出一株具有T6E(B)S.6DL中心融合(ROPT)的植物。在F-3家系中恢复了1个纯合子易位系，并用基因组原位杂交(GISH)进行了验证。籽粒微量营养元素分析表明，DS6E(B)(6D)代换系和T6E(B)S.6DL砧木的铁、锌含量均高于受体小麦品种柔山。
Development of wheat-alien translocation lines has facilitated practical utilization of alien species in wheat improvement. The production of a compensating Triticum aestivum-Thinopyrum bessarabicum whole-arm Robertsonian translocation (RobT) involving chromosomes 6D of wheat and 6E(b) of Th.bessarabicum (2n=2x=14, (EEb)-E-b) through the mechanism of centric breakage-fusion is reported here. An F-2 population was derived from plants double-monosomic for chromosome 6D and 6E(b) from crosses between a DS6E(b)(6D) substitution line and bread wheat cultivar Roushan' (2n=6x=42, AABBDD) as female parent. Eighty F-2 genotypes (L1-L80) were screened for chromosome composition. Three PCR-based Landmark Unique Gene (PLUG) markers specific to chromosomes 6D and 6E(b) were used for screening the F-2 plants. One plant with a T6E(b)S.6DL centric fusion (RobT) was identified. A homozygous translocation line with full fertility was recovered among F-3 families and verified with genomic insitu hybridization (GISH). Grain micronutrient analysis showed that the DS6E(b)(6D) substitution line and T6E(b)S.6DL stock have higher Fe and Zn contents than the recipient wheat cultivar Roushan'.