Multiple forms of calmodulin genes in carrot treated with fungal mycelial walls.
Multiple forms of calmodulin genes in carrot treated with fungal mycelial walls.
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DOI：
10.1248/bpb.28.1109
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发表时间：
2005-06
影响因子：
2
通讯作者：
Eriko Ishigaki;R. Sugiyama;F. Kurosaki
中科院分区：
文献类型：
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作者：
Eriko Ishigaki;R. Sugiyama;F. Kurosaki
从真菌菌丝壁处理过的胡萝卜幼苗中分离到11个编码钙调蛋白和钙调蛋白样蛋白开放阅读框的cDNA克隆。这些钙调素基因主要在胡萝卜茎和叶中表达，而在种子和根组织中的转录水平几乎可以忽略不计。基于核苷酸同源性，将这些基因分为I类和II类两类，逆转录-聚合酶链反应分析显示，II类基因的表达量在胡萝卜幼苗用菌丝壁处理后短暂增加。相比之下，即使在真菌成分处理后，I类基因的水平也没有明显的变化。这些结果表明，在真菌感染的胡萝卜中诱导的防御反应伴随着某种钙调素基因转录水平的增加。
Eleven cDNA clones encoding the open-reading frame of calmodulin and calmodulin-like proteins were isolated from carrot seedlings treated with fungal mycelial walls. These calmodulin genes were mainly expressed in the stems and leaves of carrot, although the transcriptional level was almost negligible in the seeds and root tissues. Based on nucleotide homology, these genes were divided into two classes, class I and class II, and reverse transcription-polymerase chain reaction analyses revealed that the expression level of class II genes transiently increased when carrot seedlings were treated with the mycelial walls. In contrast, the level of class I genes apparently did not show any significant change even after treatment with the fungal components. These results suggest that the defense responses induced in the fungi-infected carrot accompany the increase in the transcriptional levels of a certain calmodulin gene(s).