Characteristics of a cancerous cell line, HIOEC-B(a)P-96, induced by benzo(a)pyrene from human immortalized oral epithelial cell line
Characteristics of a cancerous cell line, HIOEC-B(a)P-96, induced by benzo(a)pyrene from human immortalized oral epithelial cell line
复制标题
由人永生化口腔上皮细胞系苯并(a)芘诱导的癌细胞系 HIOEC-B(a)P-96 的特征。
DOI：
10.1016/j.archoralbio.2007.12.002
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发表时间：
2008-05-01
影响因子：
3
通讯作者：
Zhang, Zhi-Yuan
中科院分区：
文献类型：
--
作者：
Zhong, Lai-Ping;Pan, Hong-Ya;Zhang, Zhi-Yuan
口腔鳞状细胞癌（OSCC）是口腔颌面部最常见的恶性肿瘤。OSCC的癌变机制尚不清楚。体外研究OSCC细胞系，特别是来源于永生化口腔上皮细胞的OSCC细胞系，是了解癌变机制的一个非常有用的策略。我们以转染HPV16 E6/E7基因获得的人永活口腔上皮细胞（HIOEC）为基础，用苯并(a)芘诱导建立了一株新的癌细胞HIOEC- b (a)P-96 （HB96）。对HB96细胞的形态、超微结构、增殖能力、侵袭能力及肿瘤发生等特征进行了研究。HB96细胞失去接触抑制，细胞分裂失控，细胞重叠明显，细胞形状呈多角形，大小不均匀，核浆比增大。细胞增殖分析和细胞侵袭实验证实了细胞增殖能力和侵袭能力的增强。裸鼠实验病理证实了高分化至中分化鳞状细胞癌的致瘤性。Western blotting也证实HPV16 E6/E7蛋白表达升高，与p53、Rb蛋白表达降低有明显相关性。因此，这种来自HIOEC系的苯并(a)芘诱导的HB96细胞系，为今后的基因组学和蛋白质组学分析提供了体外研究OSCC致癌机制的有用工具。这也是国内第一个从永活口腔上皮细胞中获得的体外OSCC癌细胞系。(c) 2007 Elsevier Ltd.版权所有。
Oral squamous cell carcinoma (OSCC) is the most common malignant tumor in the oral and maxillofacial region. The mechanism of carcinogenesis of OSCC is still unclear. In vitro study on OSCC cell lines, especially derived from immortalized oral epithelial cells, is a very useful strategy to understand the mechanism of carcinogenesis. Based on our previous human immortalized oral epithelial cell (HIOEC) line, obtained from normal oral epithelial cells by transfection of HPV16 E6/E7 gene, a new cancerous cell line, HIOEC-B(a)P-96 (HB96), was established from the HIOEC by induction with benzo(a)pyrene. The characteristics of the HB96 cells such as cell morphology, ultrastructure, proliferation ability, invasion ability, and tumorigenesis were studied. The HB96 cells lost contact inhibition with uncontrolled cell division and obvious cell overlap, they were polygonal in shape and ununiform in size with increased ratio between nucleus and plasma. Increased proliferative ability and invasion ability were confirmed by the cell proliferation analysis and cell invasion assay, respectively. The tumorigenicity of well to moderately differentiated squamous cell carcinoma was confirmed in the nude mice experiments pathologically. Increased expression of HPV16 E6/E7 proteins and obvious correlation with decreased expression of p53 and Rb proteins was also confirmed by Western blotting. Thus, this HB96 cell line induced by benzo(a)pyrene from the HIOEC line is a useful tool to study the mechanism of carcinogenesis of OSCC in vitro for future genomic and proteomic analyses. It is also the first in vitro cancerous cell line of OSCC in China derived from immortalized oral epithelial cells. (c) 2007 Elsevier Ltd. All rights reserved.