Elevated expression of interleukin-9 mRNA in the bronchial mucosa of atopic asthmatics and allergen-induced cutaneous late-phase reaction: relationships to eosinophils, mast cells and T lymphocytes
Elevated expression of interleukin-9 mRNA in the bronchial mucosa of atopic asthmatics and allergen-induced cutaneous late-phase reaction: relationships to eosinophils, mast cells and T lymphocytes
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DOI：
10.1046/j.1365-2222.2002.01376.x
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发表时间：
2002-06-01
影响因子：
6.1
通讯作者：
Robinson, DS
中科院分区：
文献类型：
--
作者：
Ying, S;Meng, Q;Robinson, DS
背景 Interleukin-9 是一种 T 细胞衍生的 Th2 型细胞因子，与气道高反应性有关。动物模型中粘液分泌过多和肥大细胞浸润。我们最近证明了 IL-9 在人类嗜酸性粒细胞发育和存活中发挥作用的潜力。 目的 本研究的目的是：(i) 比较特应性哮喘患者和正常对照者支气管活检中 IL-9 mRNA 的表达，(ii) 研究过敏原攻击后皮肤活检中 IL-9 mRNA 的动力学表达； (iii) 将 IL-9 表达与局部组织中嗜酸性粒细胞、肥大细胞和 T 淋巴细胞的浸润联系起来。 方法 在基线时从特应性哮喘患者 (n = 12) 和正常非哮喘患者 (n = 12) 获得支气管活检。在过敏原激发后 1、3、6、24、48 和 72 小时从特应性受试者 (n = 11) 获取皮肤活检。 24 小时时的稀释剂攻击位点用作对照。使用原位杂交技术鉴定IL-9 mRNA。通过免疫组织化学评估嗜酸性粒细胞、肥大细胞和T细胞的数量。结果特应性哮喘患者支气管粘膜中IL-9 mRNA细胞的数量显着高于正常对照组（P = 0.003）。嗜酸性粒细胞的数量，但不是肥大细胞的数量。哮喘患者的这一比例也显着较高（P < 0.005）。哮喘患者气道中 IL-9 mRNA 细胞的数量与嗜酸性粒细胞的数量显着相关（r = 0.623。P = 0.03），但与肥大细胞或 T 细胞无关。与稀释剂攻击相比，皮肤中所有过敏原攻击部位的 IL-9 mRNA 细胞数量均显着升高。 48 小时时信号最大（P < 0.005）。 72 小时时。 IL-9 mRNA(+)细胞数量与嗜酸性粒细胞数量显着相关(r = 0.707, P = 0.015)。 结论 过敏性炎症中IL-9表达升高可能有助于哮喘和其他过敏性特应性疾病的局部嗜酸性粒细胞浸润和生存。
Background Interleukin-9 is a T cell-derived Th2-type cytokine that has been linked to airway hyper-responsiveness. mucus hypersecretion and mast cell infiltration in animal models. We recently demonstrated the potential for IL-9 to act in human eosinophil development and survival.Objectives The aims of this study were: (i) to compare IL-9 mRNA expression in bronchial biopsies between atopic asthmatics and normal controls, (ii) to investigate kinetic expression of IL-9 mRNA in skin biopsies after allergen challenge; and (iii) to relate IL-9 expression to infiltration of eosinophils, mast cell and T lymphocytes in local tissue.Methods Bronchial biopsies were obtained from atopic asthmatics (n = 12) and normal non-asthmatics (n = 12) at baseline. Skin biopsies were obtained from atopic subjects (n = 11) at 1, 3, 6, 24, 48 and 72 h after allergen challenge. Diluent challenge sites at 24 h were used as controls. IL-9 mRNA was identified using the technique of in situ hybridization. The numbers of eosinophils, mast cells and T cells were evaluated by immunohistochemistry.Results The numbers of IL-9 mRNA-cells present in the bronchial mucosa were significantly greater in atopic asthmatics than those in normal controls (P = 0.003). The numbers of eosinophils, but not mast cells. were also significantly higher in asthmatics (P < 0.005). The numbers of IL-9 mRNA- cells present in the airway of asthmatics significantly correlated with the numbers of eosinophils (r = 0.623. P = 0.03), but not mast cells or T cells. Compared with diluent challenge, the numbers of IL-9 mRNA- cells were significantly elevated at all allergen-challenged sites in the skin. with maximal signals at 48 h (P < 0.005). At 72 h. the numbers of IL-9 mRNA(+) cells significantly correlated with the numbers of eosinophils (r = 0.707, P = 0.015).Conclusion Elevated expression of IL-9 in allergic inflammation may contribute to local eosinophil infiltration and survival in asthma and other allergic atopic diseases.