Evidence of Resistance to QoI Fungicides in Contemporary Populations of Mycosphaerella fijiensis, M. musicola and M. thailandica from Banana Plantations in Southeastern Brazil
Evidence of Resistance to QoI Fungicides in Contemporary Populations of Mycosphaerella fijiensis, M. musicola and M. thailandica from Banana Plantations in Southeastern Brazil
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DOI：
10.3390/agronomy12122952
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发表时间：
2022-12-01
期刊：
影响因子：
3.7
通讯作者：
Ceresini, Paulo C.
中科院分区：
文献类型：
--
作者：
Oliveira, Tamiris Y. K.;Silva, Tatiane C.;Ceresini, Paulo C.
黄斑叶斑病和黑斑叶斑病分别由斐济球腔菌和音乐斑球菌引起，是世界范围内最重要的香蕉叶部病害。疾病控制在很大程度上依赖于密集的杀菌剂喷雾，这增加了病原体种群对杀菌剂抗性的选择压力。本研究的主要目的是评估巴西东南部不同杀菌剂喷洒方案下从香蕉田取样的两种病原体种群对醌外抑制剂 (QoI-strobilurin) 杀菌剂的抗性水平和传播。其次，我们的目的是调查 QoI 耐药何时得到证实，是否与线粒体编码细胞色素 b 基因突变引起的靶位点改变 G143A 有关。在经杀菌剂处理的香蕉田中检测到 QoI 抗性，而在有机香蕉田中未检测到抗性。从病原体群体中取样的分离株中，共有 18.5% 对 QoI 具有抗性。还发现了新描述的 M. thailandica。它是里贝拉山谷中与叶斑病样叶斑病症状相关的第二丰富的球腔菌属物种，并且发现该病原体的 QoI 抗性水平最高。 G143A 细胞色素 b 的改变与 M. fijiensis、M. musicola 和 M. thailandica 菌株对 QoI 杀菌剂嘧菌酯和肟菌酯的抗性相关。为了减少抗药性的发展并保持 QoI 杀菌剂的功效，应实施基于综合病害管理实践的抗药性管理策略来控制叶斑病复合体。
Yellow and black Sigatoka, caused by Mycosphaerella fijiensis and M. musicola, respectively, are the most important worldwide foliar diseases of bananas. Disease control is heavily dependent on intensive fungicide sprays, which increase selection pressure for fungicide resistance in pathogen populations. The primary objective of this study was to assess the level and spread of resistance to quinone-outside inhibitors (QoI-strobilurin) fungicides in populations of both pathogens sampled from banana fields under different fungicide spray regimes in Southeastern Brazil. Secondly, we aimed to investigate when QoI resistance was confirmed if this was associated with the target-site alteration G143A caused by a mutation in the mitochondrial encoded cytochrome b gene. QoI resistance was detected in fungicide treated banana fields, while no resistance was detected in the organic banana field. A total of 18.5% of the isolates sampled from the pathogens' populations were resistant to QoI. The newly described M. thailandica was also found. It was the second most abundant Mycosphaerella species associated with Sigatoka-like leaf spot symptoms in the Ribeira Valley and the highest level of QoI resistance was found for this pathogen. The G143A cytochrome b alteration was associated with the resistance to the QoI fungicides azoxystrobin and trifloxystrobin in M. fijiensis, M. musicola and M. thailandica strains. In order to reduce resistance development and maintain the efficacy of QoI fungicides, anti-resistance management strategies based on integrated disease management practices should be implemented to control the Sigatoka disease complex.