Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in mice
Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in mice
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DOI：
10.1016/j.patbio.2015.03.002
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发表时间：
2015-06-01
影响因子：
--
通讯作者：
Dutkiewicz, J.
中科院分区：
文献类型：
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作者：
Golec, M.;Lemieszek, M. K.;Dutkiewicz, J.
导言。-过敏性肺炎(HP)是一种间质性肺部疾病，由反复接触有机粉尘引发的未解决的炎症和组织修复病理引起。本研究旨在探讨内毒素相关抗菌肽(CRIMP)、层粘连蛋白(LAM-A1)、部分Toll样受体(TLR)和趋化因子在实验性幽门螺杆菌(HP)发病中的变化。-3个月和18个月大的雌性C57BL/6J小鼠吸入了聚集性泛胞杆菌的生理盐水提取物，这是有机粉尘中常见的革兰氏阴性细菌，以其致病作用而闻名。每天重复吸入(28天)。采用ELISA法测定肺组织匀浆中CRAMP、LAM-A1、TLR2、TLR4、TLR8、趋化因子[C-X-C基序]配体CXCL9和CXCL10的浓度。-与未经处理的样本相比，吸入7天后，幼鼠和老年小鼠肺中TLR2、TLR4和CXCL9的水平都显著升高，而28天后LAM-A1和CXCL10的水平显著增加。TLR8水平仅在幼年小鼠中显著升高。仅抽筋水平显著下降。未处理的老年动物TLR8和CXCL9浓度显著高于年轻动物。-被检查的因子水平的显著变化表明它们在幽门螺杆菌的发病机制中发挥作用。(C)2015年爱思唯尔·马森公司。版权所有。
Introduction. - Hypersensitivity pneumonitis (HP) is an interstitial lung disease caused by unresolved inflammation and tissue repair pathologies triggered by repeated organic dust exposure. The aim of the study was to investigate changes in levels of the cathelicidin related antimicrobial peptide (CRAMP), laminin (LAM-A1), selected Toll-like receptors (TLR) and chemokines in experimental HP in mice.Materials and methods. - Three and 18-month-old female C57BL/6J mice underwent inhalations of the saline extract ofPantoea agglomerans cells, Gram-negative bacterium common in organic dust and known for its pathogenic impact. The inhalations were repeated daily (28 days). ELISA was used for measuring in lung tissue homogenates concentration of CRAMP, LAM-Al, TLR2, TLR4, TLR8, CXCL9 (chemokine [C-X-C motif] ligand) and CXCL10.Results. - Levels of TLR2, TLR4 and CXCL9 were significantly higher in both young and old mice lungs already after 7 days of inhalations, while significant increase of LAM-Al and CXCL10 was noted after 28 days, compared to untreated samples. TLR8 level was significantly augmented only in young mice. Only CRAMP level significantly declined. Significantly higher TLR8 and CXCL9 concentration in untreated samples were noted in old animals compared to young ones.Conclusion. - Significant alterations of the examined factors levels indicate their role in HP pathogenesis. (C) 2015 Elsevier Masson SAS. All rights reserved.