Physical exercise-associated gene expression signatures in peripheral blood
Physical exercise-associated gene expression signatures in peripheral blood
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DOI：
10.1097/jsm.0b013e31814c3e4f
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发表时间：
2007-09-01
影响因子：
2.7
通讯作者：
Rokutan, Kazuhito
中科院分区：
文献类型：
--
作者：
Kawai, Tomoko;Morita, Kyoko;Rokutan, Kazuhito
目的：为了评估对物理应激的反应，使用携带1467个应激响应互补DNA探针的原始微阵列分析外周血细胞的基因表达谱。设计：在60%VO2 max的踏车功率计上运动1小时后，在4，24和48小时分析基因表达（有氧运动）或直到筋疲力尽地点：德岛大学研究生院健康生命科学研究所，参与者：12名研究生博士本科学校的健康男性学生。结果：有氧运动和力竭运动分别引起21个和16个基因表达的短暂变化，峰值出现在运动后4h。只有2个基因对两种运动都有显著的反应。力竭运动而非有氧运动产生了二次反应，在24小时内14个基因的表达发生了显著改变。这些基因中的五个编码神经递质受体（HTR1A，CHRN132，GABRB3，GABRG3和LOC 51289）。结论：这里显示的单个基因的行为可能是客观评估急性身体应激和疲劳相关反应的信息。
Objective: To assess response to physical stress, gene expression profiles in peripheral blood cells were analyzed using an original microarray carrying 1467 stress-responsive complementary DNA probes.Design: Gene expression was analyzed at 4, 24, and 48 hours aft,-r exercising on a cycle ergometer at 60% VO2 max for 1 hour (aerobic exercise) or until exhausted (exhaustive exercise).Setting: Institute of Health Biosciences, University of Tokushima Graduate School,Participants: Twelve healthy male students of the postgraduate Dr undergraduate school.Interventions: The volunteers performed the aerobic or exhausti:Ve exercise on a cycle ergometer.Main Outcome Measurements: Detection of aerobic exerciseresponsive or exhaustive exercise-responsive genes in peripheral blood cells.Results: Aerobic and exhaustive exercise transiently changed the expression of 21 and 16 genes, respectively, with the peak at 4 hours. Only 2 genes significantly responded to both types of exercise. Exhaustive but not aerobic exercise produced a secondary response with significantly altered expression of 14 genes at 24 hours. Five of those genes encode receptors for neurotransmitters (HTR1A, CHRN132, GABRB3, GABRG3, and LOC51289).Conclusions: The behavior of the individual genes shown here may be informative to objectively assess acute physical stress and exhaustion-associated responses.