TELOMERASE IN YEAST
TELOMERASE IN YEAST
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DOI：
10.1126/science.7618104
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发表时间：
1995-07-21
期刊：
影响因子：
56.9
通讯作者：
BLACKBURN, EH
中科院分区：
文献类型：
--
作者：
COHN, M;BLACKBURN, EH
核糖核蛋白端粒酶通过复制内部 RNA 模板序列来合成端粒 DNA。分别具有规则和不规则端粒序列的卡氏酵母和酿酒酵母的端粒酶活性现已被鉴定和表征。酿酒酵母的活性需要端粒酶RNA基因TLC1，但不需要EST1基因，这两者都是体内正常端粒维持所必需的。该活动表现出较低的持续生产能力并且没有产生定期重复的产品。酿酒酵母端粒酶固有的高停滞频率可能解释了其体外特性以及该酵母的不规则端粒序列。
The ribonucleoprotein enzyme telomerase synthesizes telomeric DNA by copying an internal RNA template sequence. The telomerase activities of the yeasts Saccharomyces castellii and Saccharomyces cerevisiae-with regular and irregular telomeric sequences, respectively-have now been identified and characterized. The S. cerevisiae activity required the telomerase RNA gene TLC1 but not the EST1 gene, both of which are required for normal telomere maintenance in vivo. This activity exhibited low processivity and produced no regularly repeated products. An inherently high stalling frequency of the S. cerevisiae telomerase may account for its in vitro properties and for the irregular telomeric sequences of this yeast.