A METHOD FOR PREPARING ANALYTICALLY PURE SODIUM DITHIONITE - DITHIONITE QUALITY AND OBSERVED NITROGENASE-SPECIFIC ACTIVITIES
A METHOD FOR PREPARING ANALYTICALLY PURE SODIUM DITHIONITE - DITHIONITE QUALITY AND OBSERVED NITROGENASE-SPECIFIC ACTIVITIES
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DOI：
10.1016/0304-4165(91)90082-r
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发表时间：
1991-09-02
期刊：
影响因子：
--
通讯作者：
WEI, S
中科院分区：
文献类型：
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作者：
MCKENNA, CE;GUTHEIL, WG;WEI, S
连二亚硫酸钠（Na 2S 2 O 4）被广泛用作生物化学研究中的还原剂，但尚未获得其纯形式。 本文给出了一种简便、详细的方法，用于工业连二亚硫酸盐在厌氧条件下从0.1M NaOH-甲醇中重结晶。 通过UV光谱（A315）和元素分析，两次重结晶的连二亚硫酸盐具有99 +/-1%的纯度。 研究了连二亚硫酸盐质量对棕色固氮菌固氮酶组分（Av 1和Av 2）表观还原活性的影响。
Sodium dithionite (Na2S2O4) is widely used as a reductant in biochemical studies, but has not been available in its pure form. A convenient, detailed procedure is given for the recrystallization of commercial dithionite from 0.1 M NaOH-methanol under anaerobic conditions. Twice-recrystallized dithionite had a purity of 99 +/- 1% by UV spectroscopy (A315) and elemental analysis. The influence of dithionite quality on the apparent reduction activities of the nitrogenase components (Av1 and Av2) from Azotobacter vinelandii was investigated.