CUREing biochemistry lab monotony.
CUREing biochemistry lab monotony.
复制标题
消除生物化学实验室的单调。
DOI：
10.1021/acs.jchemed.2c00357
复制
发表时间：
2022
影响因子：
3
通讯作者：
FreemanJr,ThomasC
中科院分区：
文献类型：
--
作者：
Satusky,MatthewJ;Wilkins,Hunter;Hutson,Bryant;Nasiri,Mahfuz;King,Dillon;Erie,DorothyA;FreemanJr,ThomasC
本科生研究经验对于研究生研究环境的成功至关重要。最近，从“食谱”式实验室转向本科实验室基于课程的本科生研究体验（CURE），使大学能够提供包容性的研究体验，同时绕过课外学徒的限制。本文描述了一种高级生物化学 CURE，旨在通过研究可疑的 DNA 解旋酶为学生提供研究生水平研究的入门经验。该 CURE 旨在跨越多个学期，每个学生群体都以前几个学期的工作为基础。采用课前和课后调查来评估学生对基础技能、对课程的看法和项目所有权的信心。结果表明，实验室会议风格的背诵和海报演示的结合带来了更高的项目所有权，同时克服故障排除是一个重大挑战。此外，除了一个实例外，对每一种实验技术的信心都显着增加。基于这些结果，该 CURE 为学生提供了研究生水平研究的真实体验。
Undergraduate research experience is critical to success in postgraduate research settings. The recent movement away from “cookbook” style laboratories to course-based undergraduate research experiences (CUREs) in undergraduate laboratories has allowed universities to provide inclusive research experience while bypassing the limitations of extracurricular apprenticeships. This paper describes an upper-level biochemistry CURE designed to provide students with an introductory experience to graduate-level research by studying a suspected DNA helicase. This CURE is designed to span multiple semesters, where each student cohort builds upon the work of previous semesters. Pre- and postcourse surveys were employed to assess student confidence in bench skills, perceptions of the course, and project ownership. The results show that the incorporation of lab meeting style recitations and poster presentations led to higher project ownership, while overcoming troubleshooting was a significant challenge. Furthermore, confidence in every experimental technique increased significantly in all but one instance. Based on these results, this CURE is providing students with a realistic experience in graduate-level research.