Sublingual vaccination with fusion protein consisting of the functional domain of hemagglutinin A of Porphyromonas gingivalis and Escherichia coli maltose-binding protein elicits protective immunity in the oralcavity.
Sublingual vaccination with fusion protein consisting of the functional domain of hemagglutinin A of Porphyromonas gingivalis and Escherichia coli maltose-binding protein elicits protective immunity in the oralcavity.
复制标题
舌下接种由牙龈卟啉单胞菌血凝素 A 功能域和大肠杆菌麦芽糖结合蛋白组成的融合蛋白，可在口腔中引发保护性免疫。
DOI：
10.1111/j.1574-695x.2011.00895.x
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Yamamoto M.
中科院分区：
文献类型：
--
作者：
Yuzawa S;Kurita-Ochiai T;Hashizume T,Kobayashi R;Abiko Y;Yamamoto M.
本研究证明融合蛋白25K-HagA-MBP可诱导保护性免疫应答。融合蛋白25K-HagA-MBP由牙龈卟啉单胞菌血凝素A的25 kDa抗原区与大肠杆菌麦芽糖结合蛋白(MBP)融合而成。25K-HagA-MBP免疫小鼠可诱导高水平的抗原特异性血清Ig G和Ig A，以及唾液Ig A。高滴度的血清免疫球蛋白和免疫球蛋白A也诱导了近1年。在免疫球蛋白亚类分析中，舌下免疫25k-Haga-MBP可诱导IgG1和IgG2b抗体应答。此外，在最后一次免疫后7天，从唾液腺中检测到大量抗原特异性IgA抗体形成细胞。从颌下淋巴结(SML)分离的单个核细胞在25K-HagA-MBP的重新刺激下显示出显著的增殖水平。细胞因子反应分析表明，从SML分离的抗原特异性单个核细胞产生显著高水平的IL-4、干扰素-γ和转化生长因子-β。这些结果表明，舌下免疫25K-HagA-MBP可诱导有效的保护性免疫。牙龈感染是通过Th1型和Th2型细胞因子在口腔中产生的。
This study demonstrated that sublingual immunization with a fusion protein, 25k-hagA-MBP, which consists of a 25-kDa antigenic region of hemagglutinin A purified fromPorphyromonas gingivalisfused to maltose-binding protein (MBP) originating fromEscherichia colias an adjuvant, elicited protective immune responses. Immunization with 25k-hagA-MBP induced high levels of antigen-specific serum IgG and IgA, as well as salivary IgA. High level titers of serum IgG and IgA were also induced for almost 1 year. In an IgG subclass analysis, sublingual immunization with 25k-hagA-MBP induced both IgG1 and IgG2b antibody responses. Additionally, numerous antigen-specific IgA antibody-forming cells were detected from the salivary gland 7 days after the final immunization. Mononuclear cells isolated from submandibular lymph nodes (SMLs) showed significant levels of proliferation upon restimulation with 25k-hagA-MBP. An analysis of cytokine responses showed that antigen-specific mononuclear cells isolated from SMLs produced significantly high levels of IL-4, IFN-γ, and TGF-β. These results indicate that sublingual immunization with 25k-hagA-MBP induces efficient protective immunity againstP. gingivalisinfection in the oral cavity via Th1-type and Th2-type cytokine production.