Comparative sequence analysis of enteroaggregative Escherichia coli heat-stable enterotoxin 1 identified in Korean and Japanese Escherichia coli strains
Comparative sequence analysis of enteroaggregative Escherichia coli heat-stable enterotoxin 1 identified in Korean and Japanese Escherichia coli strains
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韩国和日本大肠杆菌菌株中鉴定的肠聚集性大肠杆菌耐热肠毒素 1 的比较序列分析
DOI：
10.1016/j.ijfoodmicro.2016.11.017
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Choi Changsun
中科院分区：
文献类型：
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作者：
Seo Dong Joo;Choi SunKeum;Jeon Su Been;Jeong Suntak;Park Hyunkyung;Lee Bog-Hieu;Kim Geun-Bae;Yang Soo-Jin;Nishikawa Yoshikazu;Choi Changsun
本研究的目的是比较8株韩国大肠杆菌和11株日本大肠杆菌的基因序列。利用商业DNA提取试剂盒从每个分离物的染色体和质粒DNA制备样品中使用常规PCR扩增astagene。依次进行PCR产物的克隆、序列分析和脉冲场凝胶电泳（PFGE）。在8名韩国人和8名日本人身上分别发现了相同的基因拷贝。从牛、猪和健康人类携带者中分离的大肠杆菌。其中，1个韩国分离株和4个日本分离株在残基16处携带停止突变。3株日本爆发菌株（V199、V638和96-127-23）携带多个astagene克隆，在第11、16、20、23、30、33和34个残基上有多个氨基酸变化。与非腹泻分离株相比，暴发分离株中astagene的克隆多样性和序列变化可能与EAST1的毒力潜力有关。
The aim of this study was to compare the sequence of theastAgene found in 8 Korean and 11 JapaneseEscherichia coliisolates. Conventional PCR was used to amplify theastAgene from the chromosomal and plasmid DNA preparation samples of each isolate using commercial DNA extraction kits. Cloning of the PCR products, sequence analysis, and pulse field gel electrophoresis (PFGE) were sequentially performed. An identical copy ofastAin each isolate were found for 8 Korean and 8 JapaneseE. colistrains isolated from bovine, porcine, and healthy human carriers. Among these, 1 Korean and 4 Japanese isolates carried a stop mutation at residue 16. Three Japanese outbreak strains (V199, V638, and 96-127-23) carried multiple clones ofastAgene with multiple amino acids changes at residues 11, 16, 20, 23, 30, 33, and 34. Compared with the non-diarrheal isolates, clonal diversity and sequence variations of theastAgene in outbreak isolates may be associated with virulence potential of EAST1.