A NOVEL IMMEDIATE-EARLY RESPONSE GENE OF ENDOTHELIUM IS INDUCED BY CYTOKINES AND ENCODES A SECRETED PROTEIN
A NOVEL IMMEDIATE-EARLY RESPONSE GENE OF ENDOTHELIUM IS INDUCED BY CYTOKINES AND ENCODES A SECRETED PROTEIN
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DOI：
10.1128/mcb.10.11.5830
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发表时间：
1990-11-01
影响因子：
5.3
通讯作者：
DIXIT, VM
中科院分区：
文献类型：
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作者：
HOLZMAN, LB;MARKS, RM;DIXIT, VM
我们先前已经描述了一组新的细胞立即早期应答基因的克隆，其在人脐静脉内皮细胞中的表达由肿瘤坏死因子α诱导。在放线菌酮的存在下。这些基因可能参与介导血管内皮对促炎细胞因子的反应。在这项研究中，我们进一步表征了这些新的基因产物之一，命名为B61。对编码B61的cDNA克隆的序列分析表明，其蛋白产物与先前描述的蛋白没有显著的同源性。Southern分析表明B61是一个进化上保守的单拷贝基因。B61主要是亲水性分子，但含有疏水性N-末端和疏水性C-末端区域。N-末端区域是典型的信号肽，这与蛋白质的分泌性质一致。预测蛋白的成熟形式由187个氨基酸残基组成，分子量为22，000。代谢标记的人脐静脉内皮细胞制备物的免疫沉淀显示，B61是一个25千道尔顿的分泌蛋白，其显著地由肿瘤坏死因子诱导。
We have previously described the cloning of a group of novel cellular immediate-early response genes whose expression in human umbilical vein endothelial cells is induced by tumor necrosis factor .alpha. in the presence of cycloheximide. These genes are likely to participate in mediating the response of the vascular endothelium to proinflammatory cytokines. In this study, we further characterized one of these novel gene products named B61. Sequence analysis of cDNA clones encoding B61 revealed that its protein product has no significant homology to previously described proteins. Southern analysis suggested that B61 is an evolutionarily conserved single-copy gene. B61 is primarily a hydrophilic molecule but contains both a hydrophobic N-terminal and a hydrophobic C-terminal region. The N-terminal region is typical of a signal peptide, which is consistent with the secreted nature of the protein. The mature form of the predicted protein consists of 187 amino acid residues and has a molecular weight of 22,000. Immunoprecipitation of metabolically labeled human umbilical vein endothelial cell preparations revealed that B61 is a 25-kilodalton secreted protein which is markedly induced by tumor necrosis factor.