The Measurement of Cerebral Blood Flow in the Rat
The Measurement of Cerebral Blood Flow in the Rat
复制标题
大鼠脑血流量的测定
DOI：
10.1161/01.str.6.6.630
复制
发表时间：
1975
期刊：
影响因子：
8.3
通讯作者：
O. Reinmuth
中科院分区：
文献类型：
--
作者：
A. Matsumoto;Y. Utsunomiya;K. Kogure;P. Scheinberg;O. Reinmuth
在70%笑气麻醉和戊巴比妥麻醉下测定大鼠脑血流量（CBF）。对凯蒂等人的Fick原理技术的应用进行了改进，利用稳定静脉输注123 μ l 30秒，此时将动物斩首并将头部冷冻在液氮中。通过0.13 ml体积的聚乙烯导管引导先前的股动脉-股静脉分流术。该导管以线圈形式通过NaI晶体阱计数器，由速率计记录系统记录动脉133 Ω浓度曲线。半球血流（HBF）的结果是：在70%氧化亚氮麻醉下，在正常二氧化碳下（Paco，38 mm Hg），86 ± 15 ml/100 gm/min;伴低碳酸血症（Paco，20 mm Hg），40 ± 5 ml/100 gm/分钟;伴有高碳酸血症（Paco，63 mm Hg），187 ± 10 ml/100 gm/min;戊巴比妥麻醉（Paco，38 mm Hg），41 ± 8 ml/100 gm/min。
Cerebral blood flow (CBF) was determined in the rat under 70% nitrous oxide anesthesia and pentobarbital anesthesia. The application of the Fick principle technique of Kety et al. was modified utilizing 123Xe infused intravenously steadily for 30 seconds, at which time the animal was decapitated and the head frozen in liquid nitrogen. A prior femoral artery to femoral vein shunt was led through a polyethylene catheter of 0.13 ml volume. This catheter passed as a coil in a Nal crystal well-counter with the arterial 133Xe concentration curve recorded by a ratemeter-recordcr system. The results of the hemispheric blood flow (HBF) were: under 70% nitrous oxide anesthesia in normocapnia (Paco, 38 mm Hg), 86 ± 15 ml/100 gm per minute; with hypocapnia (Paco, 20 mm Hg), 40 ± 5 ml/100 gm per minute; with hypercapnia (Paco, 63 mm Hg), 187 ± 10 ml/100 gm per minute; and with pentobarbital anesthesia (Paco, 38 mm Hg), 41 ± 8 ml/100 gm per minute.