Dynamic analysis of ribosome by a movie made from many three-dimensional electron-microscopy density maps
Dynamic analysis of ribosome by a movie made from many three-dimensional electron-microscopy density maps
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DOI：
10.2142/biophysico.16.0_108
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发表时间：
2019-04
影响因子：
1.5
通讯作者：
A. Matsumoto
中科院分区：
文献类型：
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作者：
A. Matsumoto
从许多3D-EM密度图中建立了包括结合分子在内的70年代核糖体的原子模型。结合分子的位置和构象是通过将它们与70年代核糖体拟合后保留的密度图上的区域进行拟合来确定的。然后，使用这些原子模型，制作了一部关于伸长周期的电影。为了确定模型在电影中出现的顺序，使用了关于结合分子和棘轮角度的知识。这部电影揭示了几个有趣的点，这些点在每个密度贴图上并不明显，这表明由许多3D-EM密度贴图制作的电影是有用的。
The atomic models of the 70S ribosome including the bound molecules were built from many 3D-EM density maps. The positions and conformations of the bound molecules were determined by fitting them to the regions in the density maps which remained after fitting the 70S ribosome. Then, using these atomic models, a movie for the elongation cycle was made. For determining the sequential order in which the models appeared in the movie, the knowledge about the bound molecules and the ratchet angles were used. The movie revealed several interesting points which were not apparent from each density map, suggesting the usefulness of a movie made from many 3D-EM density maps.