Open source acoustofluidics
Open source acoustofluidics
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DOI：
10.1039/c9lc00340a
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发表时间：
2019-07-21
期刊：
影响因子：
6.1
通讯作者：
Huang, Tony Jun
中科院分区：
文献类型：
--
作者：
Bachman, Hunter;Fu, Hai;Huang, Tony Jun
在过去的几十年中，已经开发了一系列声流装置，这些装置声称比传统的台式分析工具具有显着的优势。这些声流体设备经常被标记为“芯片实验室”;然而，许多人在限制其对实验室的依赖方面做得不够。通常，声流装置仍然需要熟练的操作员和复杂的外部设备。为了解决这些缺点，我们开发了一种低成本、可扩展和多功能的系统，用于在可听到低超声频率范围（31 Hz至65 kHz）内控制声流控装置。该系统是围绕现成的Arduino原型平台设计的，因为它具有用户友好的编码环境和广泛的开源材料网络;这些因素使我们能够创建一个能够生成高压振荡信号并控制声流体设备中微尺度流动的系统。利用已建立的开源系统，我们实现了一系列的声流体应用，涉及在便携式方式的流体和生物对象的操纵。特别是，我们使用我们的开源声流体设备来实现细胞和微生物的主动旋转，以及先前已显示出粘性样品制备潜力的声流体混合设备以便携式方式的操作。此外，使用低频弯曲波和我们的便携式系统，我们实现了基于尺寸的颗粒的声流分离。我们希望这里提出的开源平台可以为未来的声流体设备在护理点使用铺平道路，并简化这些设备的操作，使资源有限的用户能够在工作中利用声流体的好处。
Over the past several decades, a litany of acoustofluidic devices have been developed which purport to have significant advantages over traditional benchtop analytical tools. These acoustofluidic devices are frequently labeled as "labs-on-chips"; however, many do an insufficient job of limiting their dependence on the lab. Often, acoustofluidic devices still require skilled operators and complex external equipment. In an effort to address these shortcomings, we developed a low-cost, expandable, and multifunctional system for controlling acoustofluidic devices in the audible to low ultrasonic frequency range (31 Hz to 65 kHz). The system was designed around the readily available Arduino prototyping platform because of its user-friendly coding environment and expansive network of open source material; these factors enabled us to create a system capable of generating high voltage oscillatory signals and controlling microscale flows in acoustofluidic devices. Utilizing the established open source system, we achieved a series of acoustofluidic applications involving the manipulation of fluids and biological objects in a portable fashion. In particular, we used our open source acoustofluidic devices to achieve active rotation of cells and microorganisms, and operation of an acoustofluidic mixing device which has previously shown potential for viscous sample preparation, in a portable fashion. Additionally, using low frequency flexural waves and our portable system, we achieved acoustofluidic separation of particles based on size. It is our hope that the open source platform presented here can pave the way for future acoustofluidic devices to be used at the point-of-care, as well as simplify the operation of these devices to enable resource limited users to leverage the benefits of acoustofluidics in their work.