CD8 T-cell reactivity to islet antigens is unique to type 1 while CD4 T-cell reactivity exists in both type 1 and type 2 diabetes
CD8 T-cell reactivity to islet antigens is unique to type 1 while CD4 T-cell reactivity exists in both type 1 and type 2 diabetes
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DOI：
10.1016/j.jaut.2013.12.003
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发表时间：
2014-05-01
影响因子：
12.8
通讯作者：
von Herrath, Matthias
中科院分区：
文献类型：
--
作者：
Sarikonda, Ghanashyam;Pettus, Jeremy;von Herrath, Matthias
先前的横断面分析表明，CD8（+）和CD4(+) t细胞对胰岛特异性抗原的反应性在T1D受试者中比在健康供体（HD）中更为普遍。在这里，我们在10名HD患者、33名T1D患者和15名T2D患者中每月检测了t1 - d相关表位特异性CD4+ t细胞细胞因子产生和自身反应性CDS+ t细胞频率，为期一年。在受到刺激时，来自TID和T2D受试者的自身反应性CD4(+) t细胞比来自HD的细胞产生更多的ifn - γ。相比之下，只有在T1D中检测到更高频率的胰岛抗原特异性CD8(+) t细胞。这些观察结果支持了一般β细胞应激驱动自身反应性CD4+ t细胞活性的假设，而在T1D中常见的MHC I类胰岛过表达介导CDS+ t细胞扩增和更快的β细胞损失。总之，CD4(+) t细胞自身反应性似乎在T1D和T2D中都存在，而CD8(+) t细胞自身反应性是T1D所特有的。因此，自身反应性CD8（+）细胞可以作为一种更具t1d特异性的生物标志物。(c) 2013 Elsevier Ltd.版权所有。
Previous cross-sectional analyses demonstrated that CD8(+) and CD4(+) T-cell reactivity to islet-specific antigens was more prevalent in T1D subjects than in healthy donors (HD). Here, we examined T1-Dassociated epitope-specific CD4+ T-cell cytokine production and autoreactive CDS+ T-cell frequency on a monthly basis for one year in 10 HD, 33 subjects with T1D, and 15 subjects with T2D. Autoreactive CD4(+) T-cells from both TID and T2D subjects produced more IFN-gamma when stimulated than cells from HD. In contrast, higher frequencies of islet antigen-specific CD8(+) T-cells were detected only in T1D. These observations support the hypothesis that general beta-cell stress drives autoreactive CD4+ T-cell activity while islet over-expression of MHC class I commonly seen in T1D mediates amplification of CDS+ T-cells and more rapid beta-cell loss. In conclusion, CD4(+) T-cell autoreactivity appears to be present in both T1D and T2D while autoreactive CD8(+) T-cells are unique to T1D. Thus, autoreactive CD8(+) cells may serve as a more T1D-specific biomarker. (c) 2013 Elsevier Ltd. All rights reserved.