Vascular endothelial growth factor/vascular permeability factor mRNA expression in patients with chronic hepatitis C and hepatocellular carcinoma.
Vascular endothelial growth factor/vascular permeability factor mRNA expression in patients with chronic hepatitis C and hepatocellular carcinoma.
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DOI：
10.3892/ijo.14.2.353
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发表时间：
1999-02
影响因子：
5.2
通讯作者：
Katsuhiro Shimoda;M. Mori;K. Shibuta;Barbara F. Banner;Graham F. Barnard
中科院分区：
文献类型：
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作者：
Katsuhiro Shimoda;M. Mori;K. Shibuta;Barbara F. Banner;Graham F. Barnard
血管生成因子如血管内皮生长因子（VEGF）可能参与恶性肿瘤的新生血管形成。我们的目的是确定具有不同炎症严重程度的 HCC 和癌前丙型肝炎病毒 (HCV) 患者的肝脏中 VEGF mRNA 表达是否增加。通过逆转录和半定量聚合酶链式反应 (RT-PCR) 扩增在所有肝脏样本中检测到 VEGF mRNA（VEGF165、VEGF189）。三组间 VEGF mRNA 表达比率（针对甘油醛-3-磷酸脱氢酶进行校正）没有差异：脂肪性肝炎，作为非恶性非病毒对照，1. 05+/-0.35，n=8；脂肪性肝炎，作为非恶性非病毒对照，1. 05+/-0.35，n=8；慢性丙型肝炎，0.86+/-0.27，n=18；肝细胞癌，1.06+/-0.43，n=10。 VEGF mRNA表达与通过组织学活动指数估计的HCV炎症的严重程度无关：低HAI（l=10，n=10），0.93+/-0.31与0.81+/-0.24，p=ns。 HCC 肿瘤 (1.06+/-0.43) 和邻近组织 (0. 85+/-0.42) 之间的平均 VEGF 表达没有显着差异，尽管肿瘤往往比邻近非恶性组织具有更高的表达。总之，脂肪性肝炎、慢性HCV感染和HCC的所有肝脏样本均表达VEGF mRNA，VEGF mRNA可能在肝组织中均匀表达，表达水平可能与病毒感染或炎症严重程度无关。其他血管生成或血管抑制因子可能更多地参与 HCC 的血管生成。
Angiogenic factors such as vascular endothelial growth factor (VEGF) may be involved in neovascularization of malignant tumors. Our aim was to determine whether there is an increased VEGF mRNA expression in liver from patients with HCC and premalignant hepatitis C virus (HCV) with differing severity of inflammation. VEGF mRNA (VEGF165, VEGF189) was detected by reverse transcription and semi-quantitative polymerase chain reaction (RT-PCR) amplification in all liver samples. There was no difference in VEGF mRNA expression ratios (corrected for glyceraldehyde-3-phosphate dehydrogenase) among three groups: steatohepatitis, as a non-malignant non-viral control, 1. 05+/-0.35, n=8; chronic hepatitis C, 0.86+/-0.27, n=18; hepatocellular carcinoma, 1.06+/-0.43, n=10. VEGF mRNA expression was independent of the severity of HCV inflammation estimated by the histological activity index: low HAI (/=10, n=10), 0.93+/-0.31 vs. 0.81+/-0.24, p=ns. There was no significant difference in mean VEGF expression between HCC tumor (1.06+/- 0.43) and adjacent tissue (0. 85+/-0.42) although the tumors tended to have higher expression than adjacent non-malignant tissues. In conclusion, all liver samples of steatohepatitis, chronic HCV infection and HCC expressed VEGF mRNA, VEGF mRNA may be uniformly expressed in liver tissue, the level of expression is probably not related to virus infection or the severity of inflammation. Other angiogenic or angiostatic factors might be more involved in angiogenesis in HCC.