Intrinsically disordered regions of human plasma membrane proteins preferentially occur in the cytoplasmic segment
Intrinsically disordered regions of human plasma membrane proteins preferentially occur in the cytoplasmic segment
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DOI：
10.1016/j.jmb.2007.02.033
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发表时间：
2007-05-04
影响因子：
5.6
通讯作者：
Nishikawa, Ken
中科院分区：
文献类型：
--
作者：
Minezaki, Yoshiaki;Homma, Keiichi;Nishikawa, Ken
对2109个人质膜蛋白质进行了系统的内在无序（ID）区域的调查，并对脂质双层的跨膜拓扑结构进行了完整的分配。在41.0%的人蛋白质中检测到具有30个连续残基或更多的ID区域，远高于大肠杆菌内膜蛋白的相应数字（4.7%）。确定了每个膜蛋白在跨膜螺旋、结构域、ID和未分配区域方面的结构域组织以及细胞内部或外部的区别。长ID区分别占细胞内部和外部人质膜蛋白的13.3%和3.5%，表明它们优先出现在细胞质侧。我们解释这种现象反映了ID区域的细胞外侧和它们的相对丰富的细胞质侧在多细胞真核生物的普遍稀缺。(c)2007爱思唯尔有限公司保留所有权利。
A systematic survey of intrinsically disordered (ID) regions was carried out in 2109 human plasma membrane proteins with full assignment of the transmembrane topology with respect to the lipid bilayer. ID regions with 30 consecutive residues or more were detected in 41.0% of the human proteins, a much higher percentage than the corresponding figure (4.7%) for inner membrane proteins of Escherichia coli. The domain organization of each of the membrane protein in terms of transmembrane helices, structural domains, ID, and unassigned regions as well as the distinction of inside or outside of the cell was determined. Long ID regions constitute 13.3 and 3.5% of the human plasma membrane proteins on the inside and outside of the cell, respectively, showing that they preferentially occur on the cytoplasmic side. We interpret this phenomenon as a reflection of the general scarcity of ID regions on the extracellular side and their relative abundance on the cytoplasmic side in multicellular eukaryotic organisms. (c) 2007 Elsevier Ltd. All rights reserved.