Healthy ageing of cloned sheep.
Healthy ageing of cloned sheep.
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DOI：
10.1038/ncomms12359
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发表时间：
2016-07-26
影响因子：
16.6
通讯作者：
Gardner DS
中科院分区：
文献类型：
--
作者：
Sinclair KD;Corr SA;Gutierrez CG;Fisher PA;Lee JH;Rathbone AJ;Choi I;Campbell KH;Gardner DS
体细胞核移植（SCNT）产生的克隆动物的健康自其诞生以来一直受到关注;然而，没有对迟发性非传染性疾病进行详细评估。在这里，我们报告说，SCNT没有明显的有害的长期健康影响，在一个队列的13只克隆羊。我们在13只年龄（7-9岁）的克隆羊中进行肌肉骨骼评估，代谢测试和血压测量，其中包括4只来自多莉的细胞系。我们还对所有主要关节进行了放射学检查，包括膝关节，这是多利羊中受骨关节炎影响最大的关节，并将所有健康参数与5岁和6岁绵羊组以及已发表的参考范围进行了比较。尽管他们的高龄，这些克隆是正常的，胰岛素敏感和血压正常。重要的是，我们没有观察到退行性关节疾病的临床体征，除了轻度，或在一种情况下，在一些动物中度骨关节炎。我们的研究是第一个评估SCNT在大型动物中的长期健康结果。 自从第一只克隆动物多利羊诞生以来，人们就开始担心克隆可能对健康造成的长期后果。在这里，作者报告了一组13只老年克隆羊，其中包括4只用与多莉相同的细胞创造的克隆羊，发现它们很健康，似乎正常衰老。
The health of cloned animals generated by somatic-cell nuclear transfer (SCNT) has been of concern since its inception; however, there are no detailed assessments of late-onset, non-communicable diseases. Here we report that SCNT has no obvious detrimental long-term health effects in a cohort of 13 cloned sheep. We perform musculoskeletal assessments, metabolic tests and blood pressure measurements in 13 aged (7–9 years old) cloned sheep, including four derived from the cell line that gave rise to Dolly. We also perform radiological examinations of all main joints, including the knees, the joint most affected by osteoarthritis in Dolly, and compare all health parameters to groups of 5-and 6-year-old sheep, and published reference ranges. Despite their advanced age, these clones are euglycaemic, insulin sensitive and normotensive. Importantly, we observe no clinical signs of degenerative joint disease apart from mild, or in one case moderate, osteoarthritis in some animals. Our study is the first to assess the long-term health outcomes of SCNT in large animals. Since the birth of the first cloned animal, Dolly the sheep, concerns have been raised about potential long-term health consequences of cloning. Here the authors report on a cohort of 13 aged cloned sheep, including four created from the same cells as Dolly, and find they are healthy and seem to age normally.