Overexpression of the Transcription Factor AP37 in Rice Improves Grain Yield under Drought Conditions
Overexpression of the Transcription Factor AP37 in Rice Improves Grain Yield under Drought Conditions
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DOI：
10.1104/pp.109.137554
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发表时间：
2009-07-01
期刊：
影响因子：
7.4
通讯作者：
Kim, Ju-Kon
中科院分区：
文献类型：
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作者：
Oh, Se-Jun;Kim, Youn Shic;Kim, Ju-Kon
具有APETELA 2（AP 2）结构域的转录因子已经涉及植物发育和胁迫响应中涉及的各种细胞过程。在水稻（Oryza sativa）中预测的139个AP 2基因中，我们在目前的研究中确定了42个基因，这些基因由一种或多种胁迫条件诱导，包括干旱，高盐，低温和脱落酸。这42个胁迫诱导的AP 2基因的系统发育分析揭示了存在6个亚组（I-VI）具有不同的签名图案。两个基因，AP 37和AP 59，分别代表亚组I和II，进行了功能表征。这两个基因被发现诱导后2小时暴露于干旱和高盐度条件下，但不同的表达谱暴露于低温和脱落酸。在组成型启动子OsCc 1控制下，水稻中AP 37和AP 59的过表达增强了营养生长期对干旱和高盐的耐受性。仅在OsCc 1：AP 37植物中观察到对低温的耐受性增加。更重要的是，OsCc 1：AP 37植株在田间表现出显著增强的耐旱性，在严重干旱条件下比对照增产16%至57%，而在正常生长条件下没有表现出显著差异。相比之下，在正常和干旱胁迫条件下，田间OsCc 1：AP 59植物的籽粒产量与对照相比降低23%至43%。微阵列实验确定了10个和38个基因，分别由AP 37和AP 59上调，除了37个基因，这两个因素通常诱导。我们的研究结果表明，AP 37基因有可能提高水稻的耐旱性，而不会导致不良的生长表型。
Transcription factors with an APETELA2 (AP2) domain have been implicated in various cellular processes involved in plant development and stress responses. Of the 139 AP2 genes predicted in rice (Oryza sativa), we identified 42 genes in our current study that are induced by one or more stress conditions, including drought, high salinity, low temperature, and abscisic acid. Phylogenic analysis of these 42 stress-inducible AP2 genes revealed the presence of six subgroups (I-VI) with distinct signature motifs. Two genes, AP37 and AP59, representing subgroups I and II, respectively, were functionally characterized. Both genes were found to be induced upon 2 h of exposure to drought and high-salinity conditions but to differ in their expression profile upon exposure to low temperature and abscisic acid. The overexpression of AP37 and AP59 in rice under the control of the constitutive promoter OsCc1 increased the tolerance to drought and high salinity at the vegetative stage. Increased tolerance to low temperatures was observed only in OsCc1: AP37 plants. More importantly, the OsCc1: AP37 plants showed significantly enhanced drought tolerance in the field, which increased grain yield by 16% to 57% over controls under severe drought conditions, yet exhibited no significant difference under normal growth conditions. In contrast, grain yield in OsCc1: AP59 plants in the field was reduced by 23% to 43% compared with controls under both normal and drought stress conditions. Microarray experiments identified 10 and 38 genes that are up-regulated by AP37 and AP59, respectively, in addition to 37 genes that are commonly induced by both factors. Our results suggest that the AP37 gene has the potential to improve drought tolerance in rice without causing undesirable growth phenotypes.