Bone induction by biomimetic PLGA-(PEG-ASP)n copolymer loaded with a novel synthetic BMP-2-related peptide in vitro and in vivo
Bone induction by biomimetic PLGA-(PEG-ASP)n copolymer loaded with a novel synthetic BMP-2-related peptide in vitro and in vivo
复制标题
载有新型合成 BMP-2 相关肽的仿生 PLGA-(PEG-ASP)n 共聚物在体外和体内进行骨诱导。
DOI：
10.1016/j.jconrel.2010.02.016
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发表时间：
2010-06-01
影响因子：
10.8
通讯作者：
Yang, Shu-Hua
中科院分区：
文献类型：
--
作者：
Lin, Zhen-Yu;Duan, Zhi-Xia;Yang, Shu-Hua
骨形态发生蛋白 -2（BMP -2）是骨再生最重要的生长因子之一。聚乳酸 - 羟基乙酸共聚物（PLGA）作为一种用于递送治疗药物的可生物降解支架，已被深入研究。在先前的研究中，我们合成了一种新型的与BMP -2相关的肽（命名为P24），并发现它能够增强骨髓基质细胞（BMSCs）的成骨分化。本研究的目的是通过将P24掺入一种改性的PLGA -（PEG - ASP）n共聚物中来构建一种仿生复合材料，以促进骨形成。在体外，我们的结果表明PLGA -（PEG - ASP）n支架是一种用于P24持续释放的有效系统。附着在P24/PLGA -（PEG - ASP）n和PLGA -（PEG - ASP）n膜上的BMSCs明显多于附着在PLGA上的细胞，并且前两组中的细胞随后比PLGA组中的细胞增殖更旺盛。P24/PLGA -（PEG - ASP）n组中成骨标志物的表达强于PLGA -（PEG - ASP）n组和PLGA组。影像学和组织学检查、蛋白质印迹和逆转录 - 聚合酶链反应表明，与单独的PLGA -（PEG - ASP）n或明胶海绵相比，P24/PLGA -（PEG - ASP）n支架在体内能够诱导更有效的异位骨形成。结论是PLGA -（PEG - ASP）n共聚物是一种良好的P24载体，可作为P24控释的良好支架。这种新型的P24/PLGA -（PEG - ASP）n复合材料有望成为一种诱导骨再生的优良生物材料。（C）2010爱思唯尔B.V. 保留所有权利。
BMP-2 is one of the most important growth factors of bone regeneration. Polylactide-co-glycolic acid (PLGA), which is used as a biodegradable scaffold for delivering therapeutic agents, has been intensively investigated. In previous studies, we synthesized a novel BMP-2-related peptide (designated P24) and found that it could enhance the osteoblastic differentiation of bone marrow stromal cells (BMSCs). The objective of this study was to construct a biomimetic composite by incorporating P24 into a modified PLGA-(PEG-ASP)n copolymer to promote bone formation. In vitro, our results demonstrated that PLGA-(PEG-ASP)n scaffolds were shown to be an efficient system for sustained release of P24. Significantly more BMSCs attached to the P24/PLGA-(PEG-ASP)nand PLGA-(PEG-ASP)n membranes than to PLGA, and the cells in the two groups subsequently proliferated more vigorously than those in the PLGA group. The expression of osteogenic markers in P24/PLGA-(PEG-ASP)n group was stronger than that in the PLGA-(PEG-ASP)n and PLGA groups. Radiographic and histological examination, Western blotting and RT-PCR showed that P24/PLGA-(PEG-ASP)n scaffold could induce more effective ectopic bone formation in vivo, as compared with PLGA-(PEG-ASP)n or gelatin sponge alone. It is concluded that the PLGA-(PEG-ASP)n copolymer is a good P24 carrier and can serve as a good scaffold for controlled release of P24. This novel P24/PLGA-(PEG-ASP)n composite promises to be an excellent biomaterial for inducing bone regeneration. (C) 2010 Elsevier B.V. All rights reserved.