PHENOTYPIC ANALYSIS OF BOVINE LEUKOCYTE CELL-LINES INFECTED WITH THEILERIA-ANNULATA
PHENOTYPIC ANALYSIS OF BOVINE LEUKOCYTE CELL-LINES INFECTED WITH THEILERIA-ANNULATA
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DOI：
10.1016/0165-2427(93)90189-b
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发表时间：
1993-11-01
影响因子：
1.8
通讯作者：
BROWN, CGD
中科院分区：
文献类型：
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作者：
HOWARD, CJ;SOPP, P;BROWN, CGD
比较了6株感染环芽孢杆菌的细胞系中一系列表面分子的表达情况。用第二车间配制的单克隆抗体进行免疫荧光染色，流式细胞术分析。用牛外周血单核细胞在体外暴露于孢子虫产生的四种细胞系和用感染小牛在体内产生的一种细胞系在表型上相似。这些细胞系的表型分析没有显示出单核细胞或B细胞的起源，但表明它们不是来源于T细胞。从感染小牛体内产生的非克隆系是CD3+和γ / δ TCR+。结果表明，在体内感染的宿主细胞类型可能比迄今所怀疑的范围更广，并且一些细胞可能表达异常的表面分子模式。两者都可能对环虫感染的发病机制产生深远的影响。
Six cell lines that were infected with Theileria annulata were compared for expression of a range of surface molecules. Immunofluorescence staining with the panel of monoclonal antibodies formulated for the Second Workshop was analysed by flow cytometry. Four lines generated from bovine peripheral blood mononuclear cells by exposure to sporozoites in vitro and one line generated in vivo from an infected calf were phenotypically similar. Phenotypic analysis of those lines did not demonstrate a monocyte or B cell origin, but indicated they were not derived from T cells. An uncloned line generated in vivo from an infected calf was CD3+ and gamma/delta TCR+. The results indicate that a wider range of host cell types may be infected in vivo than suspected hitherto and that some of the cells may express an abnormal pattern of surface molecules. Both could have profound consequences for the pathogenesis of T. annulata infection.