Prosthetic Group of Aldehyde Dehydrogenase in Acetic Acid Bacteria Not Pyrroloquinoline Quinone
Prosthetic Group of Aldehyde Dehydrogenase in Acetic Acid Bacteria Not Pyrroloquinoline Quinone
复制标题
乙酸菌中醛脱氢酶的辅基不是吡咯并喹啉醌
DOI：
10.1271/bbb.58.2082
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
O. Adachi
中科院分区：
文献类型：
--
作者：
H. Takemura;T. Tsuchida;F. Yoshinaga;K. Matsushita;O. Adachi
通过化学诱变获得了一株产吡咯喹啉醌（pyroloquinoline quinone， PQQ）的醋酸杆菌BPR2001突变株G7。该菌株的膜结合葡萄糖脱氢酶和乙醇脱氢酶活性均存在缺陷，而这两种酶的假基均含有PQQ。然而，该突变体与亲本菌株的膜结合醛脱氢酶活性几乎相同，该酶也被认为是具有PQQ的藜蛋白。我们的研究结果表明PQQ不是膜结合醛脱氢酶的假基。
A mutant strain, G7, of Acetobacter sp. BPR2001 incapable of producing pyrroloquinoline quinone (PQQ) was obtained by chemical mutagenesis. The strain was defective in the activities of membrane-bound glucose dehydrogenase and alcohol dehydrogenase, both of which known to contain PQQ as their prosthetic group. However, the mutant had almost the same level of activity as the parental strain of membrane-bound aldehyde dehydrogenase, which also has been suggested to be a quinoprotein with PQQ. Our findings showed that PQQ was not the prosthetic group of membrane-bound aldehyde dehydrogenase.