Three-dimensional culture systems to induce chondrogenesis of adipose-derived stem cells.
Three-dimensional culture systems to induce chondrogenesis of adipose-derived stem cells.
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DOI：
10.1007/978-1-61737-960-4_15
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发表时间：
2011
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影响因子：
--
通讯作者：
Guilak, Farshid
中科院分区：
文献类型：
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作者：
Estes, Bradley T;Guilak, Farshid
干细胞可以很容易地从脂肪组织中大量获取，用于软骨修复或再生的组织工程方法。在本章中，我们描述了我们在实验室中用于诱导脂肪来源干细胞（ASC）成软骨的体外组织工程模型。除了适当的生长因子环境外，软骨形成还需要细胞在三维（3D）培养中保持圆形形态，因此生物材料支架的特性在ASC分化中也起着关键作用。还介绍了评估软骨形成的组织学和免疫组织学方法。一般来说，在这些模型系统中评估ASC软骨形成需要10-12周。
Stem cells can easily be harvested from adipose tissue in large numbers for use in tissue engineering approaches for cartilage repair or regeneration. In this chapter, we describe in vitro tissue engineering models that we have used in our laboratory for the chondrogenic induction of adipose-derived stem cell (ASC). In addition to the proper growth factor environment, chondrogenesis requires cells to be maintained in a rounded morphology in three-dimensional (3D) culture, and thus properties of the biomaterial scaffold also play a critical role in ASC differentiation. Histologic and immunohistologic methods for assessing chondrogenesis are also presented. In general, 10–12 weeks are required to assess ASC chondrogenesis in these model systems.