ENDOTHELIAL-CELL LEUKOCYTE ADHESION MOLECULE-1 (ELAM-1) AND INTERCELLULAR-ADHESION MOLECULE-1 (ICAM-1) EXPRESSION IN GINGIVAL TISSUE DURING HEALTH AND EXPERIMENTALLY-INDUCED GINGIVITIS
ENDOTHELIAL-CELL LEUKOCYTE ADHESION MOLECULE-1 (ELAM-1) AND INTERCELLULAR-ADHESION MOLECULE-1 (ICAM-1) EXPRESSION IN GINGIVAL TISSUE DURING HEALTH AND EXPERIMENTALLY-INDUCED GINGIVITIS
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DOI：
10.1111/j.1600-0765.1992.tb01746.x
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发表时间：
1992-11-01
影响因子：
3.5
通讯作者：
KINANE, DF
中科院分区：
文献类型：
--
作者：
MOUGHAL, NA;ADONOGIANAKI, E;KINANE, DF
采用免疫组织化学方法研究了21天实验性牙龈炎发作期间血管粘附分子表达和浸润白细胞数量的变化。使用针对ELAM-1（1.2B6）、ICAM-1（6.5B5）、CD 3（OKT 3- pan-T细胞）和中性粒细胞（PMN-弹性蛋白酶）的单克隆抗体来鉴定在第0、7、14和21天进行的牙龈活检中的阳性血管和白细胞。血管内皮细胞表达ELAM-1和ICAM-1在临床“健康”组织（0天）和实验发炎组织（7至21天）。阳性血管主要存在于结合上皮下的结缔组织中，其中T细胞和中性粒细胞的数量也最多。虽然T细胞被发现在所有的组织领域的研究，中性粒细胞主要集中在交界上皮和junctional结缔组织，但不存在口腔上皮区域。随着实验性牙龈炎的发展，不同组织区域中的T细胞或中性粒细胞的数量没有显著变化，尽管最强烈的血管ICAM-1和ELAM-1染色重新分布到邻近结合上皮的CT。一个突出的特点是强烈的ICAM-1阳性染色的交界上皮和它的情况下，在紧密相邻的口腔上皮，在临床上的“健康”和发炎组织。ICAM-1在结合上皮中的梯度，在龈沟方面具有最强的染色，加上在临床“健康”和发炎组织中ELAM-1和ICAM-1的血管表达，可能是指导白细胞向龈沟迁移的关键过程。
The changes in vascular adhesion molecule expression and numbers of infiltrating leukocytes during a 21-day experimental gingivitis episode were investigated immunohistochemically. Monoclonal antibodies to ELAM-1 (1.2B6), ICAM-1 (6.5B5), CD3 (OKT3 - pan-T cell) and neutrophils (PMN-elastase) were used to identify positive vessels and leukocytes within gingival biopsies taken on d 0, 7, 14 and 21. Vascular endothelium expressed ELAM-1 and ICAM-1 both in clinically 'healthy' tissue (d 0) and in experimentally inflamed tissue (d 7 to 21). Positive vessels were found mainly in the connective tissue subjacent to the junctional epithelium where the highest numbers of T cells and neutrophils were also seen. Although T cells were found in all tissue areas studied, neutrophils were largely concentrated in the junctional epithelium and the subjacent connective tissue but were absent from the oral epithelial region. As the experimental gingivitis developed, the number of T cells or neutrophils in the different tissue regions did not change significantly although the most intense vascular ICAM-1 and ELAM-1 staining redistributed to the CT adjacent to the junctional epithelium. A prominent feature was the intense ICAM-1-positive staining of the junctional epithelium and its absence in the closely adjacent oral epithelium, in both clinically 'healthy' and inflamed tissue. The gradient of ICAM-1 in junctional epithelium, with the strongest staining on the crevicular aspect plus the vascular expression of ELAM-1 and ICAM-1 in both clinically 'healthy' and inflamed tissue may be crucial processes which direct leukocyte migration towards the gingival crevice.