Hb Peterborough [beta 111(G13)Val-->Phe] in Japan; rapid identification by ESI/MS using proteolytic digests of oxidized globin.
Hb Peterborough [beta 111(G13)Val-->Phe] in Japan; rapid identification by ESI/MS using proteolytic digests of oxidized globin.
复制标题
日本 Hb Peterborough [β 111(G13)Val-->Phe]；
作者：
T. Nakanishi;A. Miyazaki;M. Kishikawa;A. Shimizu;Y. Aoki;M. Kikuchi
在一名日本女性身上发现了一种变异的血红蛋白，对她的血红蛋白进行了研究，以澄清在常规体检中发现的Hb A1c值低的原因。用电喷雾电离质谱仪对突变株进行检测和鉴定。其结构与Hb Peterborough[β111(G13)Val-->；Phe]相同。对于序列测定，氧化的珠蛋白以及未衍生的珠蛋白被胰酶和赖氨酰内肽酶切割。电喷雾电离质谱仪显示氧化珠蛋白的消化液中存在一种异常多肽。在串联质谱仪中，氧化肽中的半胱酸增加了碎片离子的丰度，有助于快速、准确地确定核心区多肽βT-12的取代。对溶血物的电喷雾电离质谱分析也显示糖化血红蛋白水平很低。患者的溶血物在异丙醇试验中显示稳定性降低。在正常Hb A的阴极侧等电聚焦检测到一条异常条带。这是Hb Peterborough的第二次报告，也是日本第一次出现这种情况。
A variant hemoglobin was found in a Japanese female whose hemoglobin was studied to clarify the cause of a low Hb A1c value, found during a routine medical examination. The detection and identification of the variant was performed by electrospray ionization mass spectrometry. Its structure was revealed to be the same as Hb Peterborough [beta 111(G13)Val-->Phe]. For sequence determination, oxidized globin as well as non-derivatized globin were cleaved by trypsin and lysyl endopeptidase. An abnormal peptide was found in digests of oxidized globin, as shown by electrospray ionization mass spectrometry. Cysteic acid in oxidized peptides enhanced the abundance of fragment ions in tandem mass spectrometry, which helped to quickly and accurately determine the substitution in beta T-12, a peptide in the core region. Electrospray ionization mass spectrometry analysis of the hemolysate also showed a low level of glycated hemoglobin. The patient's hemolysate showed decreased stability in the isopropanol test. An abnormal band was detected on isoelectrofocusing on the cathodic side of normal Hb A. This is the second report of Hb Peterborough and the first of its occurrence in Japan.
电喷雾质谱在变异血红蛋白临床诊断中的应用。
DOI：
10.1016/0378-4347(91)80576-x
发表时间：
1991
期刊：
Journal of chromatography
影响因子：
--
作者：
Shackleton,CH;Falick,AM;Green,BN;Witkowska,HE
通讯作者：
Witkowska,HE
通过组合 HPLC/电喷雾质谱法加快罕见变异血红蛋白的表征。
影响因子：
1
作者：
Witkowska,HE;Bitsch,F;Shackleton,CH
通讯作者：
Shackleton,CH
DOI：
10.1002/bms.1200160119
发表时间：
1988-10-01
期刊：
BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY
影响因子：
--
作者：
BIEMANN, K
通讯作者：
BIEMANN, K