The Abdominal‐B gene of Drosophila melanogaster: overlapping transcripts exhibit two different spatial distributions
The Abdominal‐B gene of Drosophila melanogaster: overlapping transcripts exhibit two different spatial distributions
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果蝇的 Abdominal-B 基因：重叠转录本表现出两种不同的空间分布
作者：
E. Sánchez;M. Crosby
果蝇的腹部- B （Abd - B）基因控制着后腹部的节段特征。我们在此描述了Abd‐B转录本的时空分布。在Abd‐B同源盒区域的Northern blots上，至少检测到5种RNA。我们已经确定了针对这些转录本亚群的特异性探针，并使用这些探针通过胚胎组织切片的原位杂交来研究每个亚群的分布。转录本可分为两组：一组在parassegment (PS)13中表达最多，并向前延伸至PS10；另一种仅在PS14和ps15中表达。这两种不同的表达模式对应于Abd‐B中两类突变所定义的解剖结构域。这些结果有助于解释该基因内复杂的遗传相互作用和突变的表型。
The Abdominal‐B (Abd‐B) gene of Drosophila controls the specification of segment identities in the posterior abdomen. We describe here the spatial and temporal distribution of Abd‐B transcripts. At least five RNA species are detected on Northern blots with probes within the region of the Abd‐B homeobox. We have identified probes specific for subsets of these transcripts and have used these probes to study the distribution of each subset by in situ hybridization to embryonic tissue sections. The transcripts can be divided into two groups: one group is expressed maximally in parasegment (PS)13 and extends anteriorly to PS10; the other is expressed only in PS14 and 15. These two different patterns of expression correspond to the anatomical domains defined by two classes of mutations in Abd‐B. These results help explain the complex genetic interactions and phenotypes of mutations within this gene.