Roles of the fission yeast formin for3p in cell polarity, actin cable formation and symmetric cell division
Roles of the fission yeast formin for3p in cell polarity, actin cable formation and symmetric cell division
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DOI：
10.1016/s0960-9822(01)00525-5
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发表时间：
2001-10-30
期刊：
影响因子：
9.2
通讯作者：
Chang, F
中科院分区：
文献类型：
--
作者：
Feierbach, B;Chang, F
背景：在发育过程中，对称和不对称细胞分裂都是产生适当细胞谱系所必需的。野生型粟酒裂殖酵母细胞以对称方式分裂，产生两个相似的杆状子细胞。福尔明是在细胞极性、胞质分裂以及肌动蛋白和微管细胞骨架的调节中具有保守作用的蛋白质。结果：在这里，我们鉴定并表征了一种新的粟酒裂殖酵母福尔明，for3p。 for3 Delta突变细胞以不对称方式分裂；母细胞向内侧分裂，产生一个发育成单极细胞的子细胞和一个发育成双极细胞的子细胞。两个子细胞本身都重现了相似的不对称谱系。双极模式的遗传与最近出生疤痕的遗传相关，而不是与纺锤体极体的不对称相关。 for3 Delta 突变体缺乏间期肌动蛋白电缆，并且肌动蛋白斑块和 myo52p（V 型肌球蛋白）分布离域。 for3 Delta 细胞具有正常的微管动力学和皮质相互作用，但存在微管组织缺陷和微管束数量增加。 for3p-GFP 以肌动蛋白依赖性方式定位于两个细胞尖端和细胞分裂位点。结论：for3p 是间期肌动蛋白电缆形成和微管组织所需的细胞极性因子。 for3 Delta 表型表明，即使没有功能性肌动蛋白电缆和肌动蛋白斑块的极化分布，细胞也能够以极化方式生长。 for3p 和可能的肌动蛋白电缆是确保细胞分裂对称的调节网络的一部分。
Background: Both symmetric and asymmetric cell divisions are required for the generation of appropriate cell lineages during development. Wild-type Schizosaccharomyces pombe cells divide in a symmetric fashion to produce two similar rod-shaped daughter cells. Formins are proteins with conserved roles in cell polarity, cytokinesis, and the regulation of actin and microtubule cytoskeletons.Results: Here, we identify and characterize a new S. pombe formin, for3p. for3 Delta mutant cells divide in an asymmetric manner; a mother cell divides medially to produce one daughter cell that develops into a monopolar cell and one daughter that develops into a bipolar cell. Both daughter cells recapitulate similar asymmetric lineages themselves. Inheritance of the bipolar pattern correlates with inheritance of the recent birth scar, not with asymmetry in the spindle pole bodies. for3 Delta mutants lack interphase actin cables and have delocalized actin patch and myo52p (type V myosin) distributions. for3 Delta cells have normal microtubule dynamics and cortical interactions but have defects in microtubule organization and increased numbers of microtubule bundles. for3p-GFP is localized at both cell tips in an actin-dependent manner and at the cell division site.Conclusions: for3p is a cell polarity factor required for interphase actin cable formation and microtubule organization. The for3 Delta phenotype suggests that cells are able to grow in a polarized manner even in the absence of functional actin cables and polarized distribution of actin patches. for3p and possibly actin cables are part of a regulatory network that ensures that cell divisions are symmetric.