Effect of moxibustion preconditioning on autophagy-related proteins in rats with myocardial ischemia reperfusion injury
Effect of moxibustion preconditioning on autophagy-related proteins in rats with myocardial ischemia reperfusion injury
复制标题
艾灸预处理对心肌缺血再灌注损伤大鼠自噬相关蛋白的影响
DOI：
10.21037/atm.2019.09.66
复制
发表时间：
2019-10-01
影响因子：
--
通讯作者：
Lu, Sheng-Feng
中科院分区：
文献类型：
--
作者：
Zhang, Hong-Ru;Bai, Hua;Lu, Sheng-Feng
背景资料：自噬在心肌缺血再灌注损伤（MIRI）的发病机制中起着重要作用。艾灸是中医常用的一种热疗法，已被证明具有心脏保护作用。然而，艾灸的心脏保护作用是否与自噬的调节有关尚不清楚。方法：雄性SD大鼠随机分为艾灸预处理组和自噬抑制剂3-甲基腺嘌呤（3-MA）干预组。结扎冠状动脉左前降支（LAD）30 min，再灌注4 h，建立MIRI模型。再灌注4 h后，采用Evans蓝和TTC染色法测定心肌梗死面积，ELISA法测定血清cTnT和乳酸脱氢酶（LDH）水平。进行Ilematoxylin和伊红（I I& E）染色用于心室组织的形态学评价。结果：莫西沙星预处理可明显减少缺血再灌注损伤后的坏死面积，心肌组织中cTnT和LDH水平与3-MA组相似，MIRI组细胞形态学改变明显减轻。同时，MIRI可上调Bedin 1和Akt的mRNA和蛋白表达，下调Bet-2的mRNA和蛋白表达。结论：在PC6处，莫昔洛韦预处理可减轻MIRI心肌损伤，其作用机制与3-MA干预相似。艾灸预处理的这种心肌保护作用可能是通过调节Bedin 1、Bcl-2和Akt的表达来调节自噬而实现的。
Background: Autophagy has increasingly been recognized as playing an essential role in the pathogenesis of myocardial ischemia reperfusion injury (MIRI). Moxibustion, a form of heat therapy commonly used in traditional Chinese medicine (TCM), has been shown to exhibit cardioprotective effects. However, whether the cardioprotective effect of moxibustion is related to the regulation of autophagy remains unknown. This study aimed to investigate the possible mechanism underlying the cardioprotective effect of moxibustion preconditioning at PC6 on MIRI by measuring the expressions of proteins involved in the regulation of autophagy.Methods: Male Sprague-Dawley rats were randomly divided to receive moxibustion preconditioning or autophagy inhibitor 3-Methyladenine (3-MA) intervention. Then the MIRI model was established by ligating the left anterior descending (LAD) coronary artery for 30 minutes followed by reperfusion for 4 hours. After 4 hours of reperfusion, the myocardial infarction area was assessed using Evans blue and TTC staining, and cTnT and lactate dehydrogenase (LDH) levels in the serum were determined by ELISA. Ilematoxylin and eosin (I I& E) staining was performed for morphological evaluation of ventricular tissues. Expressions of autophagy components Beclin 1, Bcl-2, and Akt were assessed using quantitative real-time PCR, immunohistochemistry (IHC) and western blot.Results: Moxibustion preconditioning significantly reduced the necrotic area and the levels of cTnT and LDH were similar to the 3-MA intervention, also attenuated morphological alterations were induced by MIRI. Simultaneously, the mRNA and protein expressions of Bedin 1 and Akt were up-regulated, while those of Bet-2 were down-regulated by MIRI. Moxibustion preconditioning and 3-MA intervention reversed MIRI-induced changes in Beclin 1, Akt, and Bcl-2 expressions.Conclusions: Moxibustion preconditioning at PC6 can attenuate myocardial injury for MIRI in a similar way to 3-MA intervention. This cardioprotective effect of moxibustion preconditioning may be mediated by modulating autophagy via regulation of Bedin 1, Bcl-2 and Akt.