Specific Recognition of Human Influenza Virus with PEDOT Bearing Sialic Acid-Terminated Trisaccharides
Specific Recognition of Human Influenza Virus with PEDOT Bearing Sialic Acid-Terminated Trisaccharides
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DOI：
10.1021/acsami.7b02523
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发表时间：
2017-04-26
影响因子：
9.5
通讯作者：
Miyahara, Yuji
中科院分区：
文献类型：
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作者：
Hai, Wenfeng;Goda, Tatsuro;Miyahara, Yuji
当赋予分子识别元素时，导电聚合物是生物传感器应用的良好候选者。我们开发了三糖接枝导电聚合物，用于无标记检测人类甲型流感病毒（H1N1），具有高灵敏度和特异性。带有乙氧基胺部分的3,4-乙撑二氧噻吩(EDOT)衍生物与EDOT进行电化学共聚。通过循环伏安法、X射线光电子能谱、扫描电子显微镜、触针表面轮廓仪和交流阻抗谱对所得薄膜进行了表征。将包含Sia-α 2,6'-Gal-Glu（2,6-唾液酸乳糖）或Sia-α 2,3'-Gal-Glu（2,3唾液酸乳糖）的三糖共价引入导电聚合物的侧链作为病毒识别的配体。通过石英晶体微天平（QCM）和水接触角测量证实了唾液酸乳糖的固定化。通过 QCM 和电位测定法检测人甲型流感病毒 (H1N1) 包膜中 2,6-唾液酸乳糖与血凝素的特异性相互作用，与市售试剂盒相比，灵敏度提高了 2 个数量级。所开发的具有特定病毒识别能力的导电聚合物由于其可加工性和与印刷相结合的大规模生产能力，是可穿戴监测和护理点测试的良好候选材料
Conducting polymers are good candidates for biosensor applications when molecular recognition element is imparted. We developed trisaccharide-grafted conducting polymers for label-free detection of the human influenza A virus (H1N1) with high sensitivity and specificity. A 3,4-ethylenedioxythiophene (EDOT) derivative bearing an oxylamine moiety was electrochemically copolymerized with EDOT. The obtained film was characterized by cyclic voltammetry, X-ray photoelectron spectroscopy, scanning electron microscopy, stylus surface profilometer, and AC-impedance spectroscopy. The trisaccharides comprising Sia-alpha 2,6'-Gal-Glu (2,6-sialyllactose) or Sia-alpha 2,3'-Gal-Glu (2,3sialyllactose) were covalently introduced to the side chain of the conducting polymers as a ligand for viral recognition. Immobilization of sialyllactose was confirmed by quartz crystal microbalance (QCM) and water contact angle measurements. Specific interaction of 2,6-sialyllactose with hemagglutinin in the envelope of the human influenza A virus (H1N1) was detected by QCM and potentiometry with enhanced sensitivity by 2 orders of magnitude when compared with that of commercially available kits. The developed conducting polymers possessing specific virus recognition are a good candidate material for wearable monitoring and point-of-care testing because of their processability and mass productivity in combination with printing