Size, complexity and abundance of a specific poly(A)-containing RNA of liver from male Xenopus induced to vitellogenin synthesis by estrogen.
Size, complexity and abundance of a specific poly(A)-containing RNA of liver from male Xenopus induced to vitellogenin synthesis by estrogen.
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雄性非洲爪蟾肝脏中特定的含有多聚腺苷酸 (poly(A)) 的 RNA 的大小、复杂性和丰度，由雌激素诱导卵黄蛋白原合成。
DOI：
10.1111/j.1432-1033.1976.tb10570.x
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
G. Ryffel
中科院分区：
文献类型：
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作者：
Walter Wahli;T. Wyler;Rudolf Weber;G. Ryffel
在雌激素诱导的卵黄前体蛋白卵黄原蛋白大量产生的阶段，雄性非洲爪哇的雌激素处理会导致肝脏中出现一种新的含有聚(A)的RNA。这种雌激素诱导的核糖核酸在28 S处沉淀，并在表观分子量为2.0×10(6)的水溶液中迁移到凝胶上。在电子显微镜下变性条件下的轮廓长度测量表明，与小鼠28-S rRNA相比，其相对分子质量为2.34×10(6)。标记实验表明，雌激素诱导的RNA比平均的含多聚(A)的RNA更稳定，标记24小时后，其占细胞质中含多聚(A)的RNA的10-20%。在分离的雌激素诱导的RNA上合成的互补DNA与其模板杂交表明，与含有如此高分子量的单个种类的聚(A)的RNA相对应的复杂性。将互补DNA与雌激素处理的非洲爪哇雄性和对照蟾蜍的细胞质含Poly(A)的RNA进行杂交，结果表明，雌激素诱导的RNA占所有含Poly(A)的细胞质RNA的12%-15%，而在未处理的雄性中，雌激素诱导的RNA的含量至少低2000倍。雌激素诱导的RNA的大小、复杂性和丰度是编码卵黄蛋白原的信使核糖核酸的特征。
Estrogen treatment of Xenopus males leads to the appearance of a new species of poly (A)-containing RNA in the liver, at a stage when large amounts of the estrogen-induced yolk precursor protein, vitellogenin, is produced. This estrogen-induced RNA sediments at 28 S and migrates on gels in aqueous solution with an apparent molecular weight of 2.0 X 10(6). Contour length measurements under denaturing conditions in the electron microscope reveal a molecular weight of 2.34 X 10(6) compared to the mouse 28-S rRNA. Labeling experiments show that the estrogen-induced RNA has a stability than the average liver poly(A)-containing RNA and represents 10-20% of the poly(a)-containing RNA in the cytoplasm after 24 h of labeling. Hybridization of complementary DNA, synthesized on the isolated estrogen-induced RNA, with its template suggests a complexity corresponding to a single species of poly(A)-containing RNA of such a high molecular weight. Hybridization of the complementary DNA with cytoplasmic poly (A)-containing RNA from estrogen-treated Xenopus males and control toads show that the estrogen-induced RNA constitutes 12-15% of all cytoplasmic poly(A)-containing RNA, and is at least 2000-fold less abundant in untreated males. Size, complexity and abundance of the estrogen-induced RNA are characteristics expected for a mRNA coding for vitellogenin.